%0 Journal Article
%T PI(4,5)P2 determines the threshold of mechanical force¨Cinduced B cell activation
%A Chen
%A Chenguang
%A Chenguang Xu
%A Fei
%A Fei Wang
%A Haodong
%A Haodong Chen
%A Hengyi
%A Hengyi Xie
%A Ji
%A Ji-Shen
%A Ji-Shen Zheng
%A Jing
%A Jing Wang
%A Jizhong
%A Jizhong Lou
%A Li
%A Liu
%A Lou
%A Qinghua
%A Qinghua Ji
%A Samina
%A Samina Shaheen
%A Shaheen
%A Tang
%A Wan
%A Wang
%A Wanli Liu
%A Wei
%A Wei Chen
%A Xiangjun
%A Xiangjun Chen
%A Xie
%A Xingyu
%A Xingyu Ji
%A Xu
%A Yang
%A Yang Xu
%A Zheng
%A Zhengpeng
%A Zhengpeng Wan
%A Zhuo
%A Zhuo Tang
%A Zongyu
%A Zongyu Li
%J JCB | The Journal of Cell Biology
%D 2018
%R 10.1083/jcb.201711055
%X B lymphocytes use B cell receptors (BCRs) to sense the chemical and physical features of antigens. The activation of isotype-switched IgG-BCR by mechanical force exhibits a distinct sensitivity and threshold in comparison with IgM-BCR. However, molecular mechanisms governing these differences remain to be identified. In this study, we report that the low threshold of IgG-BCR activation by mechanical force is highly dependent on tethering of the cytoplasmic tail of the IgG-BCR heavy chain (IgG-tail) to the plasma membrane. Mechanistically, we show that the positively charged residues in the IgG-tail play a crucial role by highly enriching phosphatidylinositol (4,5)-biphosphate (PI(4,5)P2) into the membrane microdomains of IgG-BCRs. Indeed, manipulating the amounts of PI(4,5)P2 within IgG-BCR membrane microdomains significantly altered the threshold and sensitivity of IgG-BCR activation. Our results reveal a lipid-dependent mechanism for determining the threshold of IgG-BCR activation by mechanical force.
%U http://jcb.rupress.org/content/217/7/2565