%0 Journal Article
%T Production of Monoclonal Antibodies against Common Epitopes of Salmonella by Oral Immunization of Laboratory Animals | Insight Medical Publishing
%A Haralambieva I
%A Iankov I
%A Mangarov I
%A Mitov I
%A Pavlova M
%A Velev V
%J (2 years) : 0.307*
%D 2018
%R 10.21767/2393-8854.100030
%X Materials and Methods Twelve mice were divided into two groups and immunized orally with S. suberu (3.10: g, m) and S. strasburg from the OD2 group (O:9.46). The K£¿hler and Milstein method yielded IgA and IgM class IgA secreting monoclonal antibodies. They were characterized by the methods of slide-agglutination, haemagglutination, ELISA, immunoblotting. Results Four anti-flagelar MAbs of class IgA and one anti-O-class IgM class were obtained. All of the IgA class reacted with the Hg epitope of flags obtained from Salmonella strains and S. enteritidis flagellin. In IgMMAbs, only O-antigens isolated from Salmonella strains belonging to the OD2 group were present. Conclusion Through this method of oral immunization, besides highly specific IgA MAbs, we also received a clone of hybridomas expressing specific IgM MAbs which by ELISA and SAT show excellent opportunity to participate in diagnostic reactions against salmonellae with a common group epitope OD2.
%U http://www.imedpub.com/articles/production-of-monoclonal-antibodies-against-common-epitopes-of-salmonella-by-oral-immunization-of-laboratory-animals.php?aid=21872