%0 Journal Article
%T A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: pharmacokinetic outcomes | Bioanalysis
%A Ahmed F El-Yazbi
%A Eman I El-Kimary
%A Essam F Khamis
%A Fawzy A El-Yazbi
%A Mohammed AW Elkhatib
%A Omayma A Amin
%A Sameh E Younis
%J Bioanalysis
%D 2019
%R https://doi.org/10.4155/bio-2018-0219
%X Aim: To develop a simple HPLC-DAD method for simultaneous determination of febuxostat (FEB) and diclofenac (DIC) in biological samples to assess pharmacokinetic outcomes of their coadministration. Methodology & results: Sample preparation was performed by liquid¨Cliquid extraction. Drugs analysis was done on C18 column using methanol-formic acid pH 2.1 (76:24, v/v) as mobile phase and time-programmed UV detection. Lower limits of quantitation for FEB and DIC were 10 and 20 ng/ml, respectively. Baseline pharmacokinetics were similar to published data on either drug alone. Coadministration led to more than twofold increase in FEB Cmax and AUC together with a reduced hepatic uptake in rats. Conclusion: DIC interfered with initial distribution and terminal clearance of FEB potentially due to reduced FEB hepatic uptake.
%U https://www.future-science.com/doi/10.4155/bio-2018-0219