%0 Journal Article
%T Juvenile rat and pediatric trazodone studies: how to gain extra sensitivity to overcome bioanalytical challenges | Bioanalysis
%A Anna Alfieri
%A Carolina Devastato
%A Fabio Garofolo
%A Graziella Bovi
%A Laura Oggianu
%A Patrizia Dragone
%A Rossella Picollo
%A Serena Tongiani
%A Silvana Olivieri
%A Vanessa Petrucci
%J Bioanalysis
%D 2019
%R https://doi.org/10.4155/bio-2018-0236
%X Aim: Trazodone (TZD) is used for the treatment of depression in adults and, off-label, as a sleep medication in adult and pediatric populations. The off-label use is well documented, however further clinical studies are needed to confirm its efficacy and safety for the treatment of sleep disorders. In this scenario, we developed a bioanalytical method to quantify low TZD concentrations in samples collected by capillary microsampling (CMS) to support dose finding, Good Laboratory Practice juvenile rat toxicokinetic and upcoming pediatric studies. Methodology: A method using only 8 ¦Ěl of plasma was developed and successfully used for analyzing CMS samples from juvenile rats throughout toxicokinetic study. Conclusion: By harmoniously maximizing each analytical step, we achieved a sensitive method to quantify TZD in CMS samples.
%U https://www.future-science.com/doi/10.4155/bio-2018-0236