%0 Journal Article
%T KSHV induces immunoglobulin rearrangements in mature B lymphocytes
%A Amy Chadburn
%A Ethel Cesarman
%A Geoffrey Mikita
%A Jennifer Totonchy
%A Jessica M. Osborn
%A Lissenya Argueta
%A Ramina Nabiee
%J -
%D 2018
%R 10.1371/journal.ppat.1006967
%X Kaposi sarcoma herpesvirus (KSHV/HHV-8) is a B cell tropic human pathogen, which is present in vivo in monotypic immunoglobulin ¦Ë (Ig¦Ë) light chain but polyclonal B cells. In the current study, we use cell sorting to infect specific B cell lineages from human tonsil specimens in order to examine the immunophenotypic alterations associated with KSHV infection. We describe IL-6 dependent maturation of na£¿ve B lymphocytes in response to KSHV infection and determine that the Ig¦Ë monotypic bias of KSHV infection in vivo is due to viral induction of BCR revision. Infection of immunoglobulin ¦Ê (Ig¦Ê) na£¿ve B cells induces expression of Ig¦Ë and isotypic inclusion, with eventual loss of Ig¦Ê. We show that this phenotypic shift occurs via re-induction of Rag-mediated V(D)J recombination. These data explain the selective presence of KSHV in Ig¦Ë B cells in vivo and provide the first evidence that a human pathogen can manipulate the molecular mechanisms responsible for immunoglobulin diversity
%K B cells
%K Lymphocytes
%K Tonsils
%K Antibodies
%K Polymerase chain reaction
%K Kaposi's sarcoma-associated herpesvirus
%K Reverse transcriptase-polymerase chain reaction
%K RNA extraction
%U https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006967