%0 Journal Article
%T Molecular characterization of Vibrio cholerae responsible for cholera epidemics in Uganda by PCR, MLVA and WGS
%A Amanda Kay Debes
%A Ambrose Wabwire Buyinza
%A Atek Kagirita
%A Christopher Garimoi Orach
%A David A. Sack
%A Godfrey Bwire
%A Joseph B. Voeglein
%A Mathieu Almeida
%A O. Colin Stine
%A Shan Li
%J -
%D 2018
%R 10.1371/journal.pntd.0006492
%X Background For almost 50 years sub-Saharan Africa, including Uganda, has experienced several outbreaks due to Vibrio cholerae. Our aim was to determine the genetic relatedness and spread of strains responsible for cholera outbreaks in Uganda.   Methodology/Principal findings Sixty-three V. cholerae isolates collected from outbreaks in Uganda between 2014 and 2016 were tested using multiplex polymerase chain reaction (PCR), multi-locus variable number of tandem repeat analysis (MLVA) and whole genome sequencing (WGS). Three closely related MLVA clonal complexes (CC) were identified: CC1, 32% (20/63); CC2, 40% (25/63) and CC3, 28% (18/63). Each CC contained isolates from a different WGS clade. These clades were contained in the third wave of the 7th cholera pandemic strain, two clades were contained in the transmission event (T)10 lineage and other in T13. Analysing the dates and genetic relatedness revealed that V. cholerae genetic lineages spread between districts within Uganda and across national borders.   Conclusion The V. cholerae strains showed local and regional transmission within Uganda and the East African region. To prevent, control and eliminate cholera, these countries should implement strong cross-border collaboration and regional coordination of preventive activities
%K Vibrio cholerae
%K Cholera
%K Uganda
%K Africa
%K Infectious disease control
%K Polymerase chain reaction
%K Lakes
%K Tanzania
%U https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006492