%0 Journal Article
%T Microrna Profiling of Differentiated, Poorly Differentiated and Anaplastic Thyroid Carcinoma, A Comparative Approach - Microrna Profiling of Differentiated, Poorly Differentiated and Anaplastic Thyroid Carcinoma, A Comparative Approach - Open Access Pub
%A Ashraf Khan
%A Madhu P Menon
%A Sarah Tadiri
%A Stefan Schattgen
%J OAP | Home | Journal of Cancer Genetics And Biomarkers | Open Access Pub
%D 2017
%X Thyroid carcinomas encompass a wide spectrum ranging from differentiated thyroid carcinoma (DTC) to poorly differentiated (PDC) and anaplastic thyroid carcinoma (ATC). DTC of both follicular (FTC) and papillary (PTC) types can progress to PDC and AC. The aim of our study was to evaluate if there is differential microRNA (miRNA) expression in various tumor subtypes during this progression. The miRNA profile of differentiated carcinomas (Follicular and Papillary) and ATC were compared with that of PDCs either by itself or in a background of differentiated carcinomas and anaplastic carcinomas. Unsupervised hierarchical clustering analysis revealed that FTC and PDC tend to cluster together in the absence of ATC. Interestingly, in cases with presence of all components i.e. FTC, PDC and ATC, the miRNA profile of poorly differentiated component clusters with that of the Anaplastic carcinoma component. miR-494 and miR-125a-5p were found to be differentially regulated in tumors with an anaplastic component and even the well-differentiated component (FTC) of these tumors were found to be aligned with the anaplastic profile. In addition, we also discovered some differentially regulated miRNAs in follicular variant of papillary thyroid carcinoma as compared to follicular thyroid carcinoma (miR-486-5p and miR-31). DOI10.14302/issn.2572-3030.jcgb-14-495 Micro-RNAs (miRNAs) are small non-coding RNAs of approximately 21-25 nucleotides that negatively regulate gene expression 1, 2, 3. It is one of the largest gene families accounting for approximately 1% of the genome and regulate approximately 1/3rd of all human genes 4, 5, 6. Micro-RNAs have been demonstrated to have multiple roles in regulating embryonic development, proliferation, apoptosis, etc. It has also emerged as an important modulator of oncogenesis and have been demonstrated to be deregulated in various hematological neoplasms, prostate cancer, thyroid neoplasms, lung cancer, pancreatic and colo-rectal tumors etc 5. Thyroid carcinoma, which constitutes more than 98% of thyroid malignancies accounts for 1% of all human cancers 7. Differentiated thyroid carcinoma (DTC) of both follicular (FTC) and papillary type (PTC) may progress to poorly differentiated carcinoma (PDC) which is a heterogeneous group that may either present as insular carcinoma or a group of less well-defined morphologic phenotype. It is not uncommon to see foci of insular growth associated with differentiated thyroid carcinoma of both papillary and follicular types. Yamashita et al. in a series of 82 follicular carcinoma reported presence of
%U https://www.openaccesspub.org/jcgb/article/163