%0 Journal Article
%T Human Umbilical Cord-derived Mesenchymal Stem Cells As A Source of Odontoblasts: A Gmp-compliant Approach - Human Umbilical Cord-derived Mesenchymal Stem Cells As A Source of Odontoblasts: A Gmp-compliant Approach - Open Access Pub
%A Antonio Marmotti
%A Brigitte Bisaro
%A Cristina Zanini
%A Federico Mussano
%A Marco Forni
%A Silvia Mattia
%J OAP | Home | Journal of Evolving Stem Cell Research | Open Access Pub
%D 2017
%X Stem cell-based regenerative therapy can be considered an innovative approach for curing dental caries. Pulp stem cells from human exfoliated deciduous teeth (SHEDs) represent a source of committed cells for generating odontoblasts in vitro; however, SHEDs are not easy to obtain and are limited in quantity. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are considered to be adult stem cells that can be easily obtained in large numbers. Here, SHEDs and UC-MSCs were conditioned in custom-made serum-free culture media in order to induce differentiation towards odontoblasts. SHEDs and UC-MSCs were expanded in vitro and differentiated into odontoblasts for 21 days using a medium containing transforming growth factor-¦Â (TGF-b3), hepatocyte growth factor (HGF) and growth differentiation factor 5 (GDF5). The ability to induce odontoblast differentiation with a straightforward clinical protocol in compliance with good manufactoring practice (GMP), which avoids animal reagents, and uses unrelated stem cells of unrestricted availability, may be a first step towards a new innovative approach for dentin regeneration. DOI10.14302/issn.2574-4372.jesr-16-1327 The treatment protocol of penetrating caries involves root canal treatment, which removes the inflamed tissue and nociceptive nerve endings, ensures pain sedation and prevents outbreaks of infection with undesirable outcomes on the overall health. The loss of the pulp, however, also results in the failure to supply nutrients and oxygen to the dentin, with consequent modification of the mechanical characteristics of the tooth which, no longer perfused, becomes fragile. To this end, the prosthetic restoration of teeth- especially premolars and molars- that have received endodontic treatments is highly recommended for preservation purposes. Like all intraoral appliances, the fixed prosthesis constructed as such is subjected to a life cycle of about 20 years, on average, and requires additional treatment in the majority of patients. Stem cells 1 are a cell population which has unique characteristics compared to differentiated cells (the possibility of self-renewal, proliferative capacity, and the ability to differentiate into most cell types). Mesenchymal stem cells (MSCs) can give rise to the entire range of connective tissues, from osteoblasts to chondrocytes, from adipocytes to myoblasts and represent a particularly interesting target due to their potential use in regenerative therapy 2, 3. The main source of MSCs is from the bone marrow, which contains the greatest amount of MSCs, but is not always easily
%U https://www.openaccesspub.org/jesr/article/412