%0 Journal Article
%T A New Spectrofluorimetric Approach For the Quantitation of Imipramine Hcl in Commercial Dosage Forms - A New Spectrofluorimetric Approach For the Quantitation of Imipramine Hcl in Commercial Dosage Forms - Open Access Pub
%A Ibtihal Mohammed Al-Ajmi
%A Manal Khalifa Al-Hattali
%A Ruiyah Khalifa Al-Hinai
%A Syed Najmul Hejaz Azmi
%J OAP | Home | Journal of Advanced Pharmaceutical Science And Technology | Open Access Pub
%D 2018
%X A spectrofluorimetric method has been developed for the determination of imipramine HCl in bulk and commercial dosage forms. The method was based on measuring the fluorescence emission intensity of imipramine-eosin Y ion pair complex (¦Ëem = 558 and ¦Ëex = 319) in dichloroethane at buffer solution (sodium acetate and acetic acid) of pH 4.8. The stoichiometric ratio between imipramine and eosin Y was studied by Job's method of continuous variations and found to be 2:1. Formation constant (Kf) and Gibb¡¯s free energy change (¦¤G) were calculated and pointed towards the spontaneous nature of the reaction. A series of variables were studied to optimize the reaction conditions. The proposed method was validated as per ICH guidelines and successfully applied for the determination of active imipramine HCl in commercial dosage forms with high degree of accuracy and precision. DOI10.14302/issn.2328-0182.japst-17-1714 Imipramine hydrochloride is chemically known as 5-3-(dimethylamino)propyl-10,11-dihydro-5H-dibenzbf-azepine monohydrochloride (Chemical Abstract Service: 113-52-0; Molecular Weight: 316.88). The drug is a dibenzazepine derivative of tricyclic antidepressant, competitively blocks the reuptake of norepinephrine and serotonin in synapses in brain 1. The drug is prescribed in the treatment of psychiatric patients suffering from depression. The drug has many variety of side effects which include drowsiness, convulsions, respiratory disorders, ophthalmoplegia, and finally coma 2. Therefore, lower dosages of imipramine hydrochloride are recommended. The inactive ingredients of imipramine are calcium phosphate, cellulose compounds, docusate sodium, iron oxides, magnesium stearate, polyethylene glycol, povidone, sodium starch glycolate, sucrose, talc, and titanium dioxide. The drug is always administered orally. It is advisable to initiate treatment at a dose of 10-25 mg daily. Toxic-effects can be induced especially when overdosed and/or high-dose drugs is combined with alcohol 3. With increasing regulatory strictness, the quality, quantity and safety of imipramine can be maintained for obtaining optimum therapeutic concentration and for quality assurance in pharmaceutical formulations. The importance of analytical techniques involved in the quality control analysis of active drug in pharmaceutical formulations has been discussed in published papers 4, 5, 6. Imipramine HCl is officially listed in British Pharmacopoeia 7 which describes a liquid chromatographic method for its assay in bulk and tablet forms. Several other analytical methods have been reported
%U https://www.openaccesspub.org/japst/article/582