%0 Journal Article
%T Discovery and Quantification in Mass Spectrometry-based Proteomics - Discovery and Quantification in Mass Spectrometry-based Proteomics - Open Access Pub
%A Cristian Ruse
%A Emily I. Chen
%J OAP | Home | Journal of Proteomics and Genomics Research | Open Access Pub
%D 2018
%X Mass spectrometry (MS) has been successfully used to analyze biological samples and advances of MS-based approaches have turn MS data from largely qualitative to quantitative. These MS-based quantitative approaches using label-free, tags, or stable isotope labeling have their own strengths and limitations. The variability introduced by different methods prior to quantitative mass spectrometry should be considered, and accuracy and precision of MS measurements can also vary depending on the strategy used for MS quantification. Therefore, the development of methods for accurate protein quantitation is one of the most challenging areas of proteomics. Using these quantitative approaches, one can investigate the dynamics of proteome through differential protein expression in normal biological processes and diseases. DOI10.14302/issn.2326-0793.jpgr-13-357 Dr. Chen received her Ph.D. degree from the Department of Molecular Pathology at the University of California, San Diego in 2002. Then, she pursued her postdoctoral training of mass spectrometry-based proteomics technology at the Scripps Research Institute in Dr. John Yate¡¯s laboratory. In 2008, she joined SUNY Stony Brook University as the scientific director of the Proteomics Center at the School of Medicine. Currently, she is the director of the Proteomics Shared Resource at the Herbert Irving Comprehensive Cancer Center and a faculty member in the Department of Pharmacology at the Columbia University Medical Center. She has been developing and conducting multidisciplinary research projects to bridge the knowledge between biology and mass spectrometry. Using the cutting-edge mass spectrometry-based proteomic techniques, she has been focused on elucidating mechanisms of tissue-specific breast cancer metastasis and identifying protein targets to eradicate the growth of distal breast cancer metastasis. She has also implemented new proteomics techniques to perform quantitative analysis on complex biological samples such as tissues from transgenic disease models and human tissues for biomarker discovery. In collaborations with other investigators, Dr. Chen applied discovery-based proteomics analysis to interrogate normal cellular processes such as regulatory mechanisms of iPSCs and post-translational modifications on pluripotent stem cells as well as disease processes such as heterogeneity of tumor microenvironment and neurodegeneration. Mass spectrometry (MS), with its qualitative and quantitative capabilities, has proven to be a power tool for biological and biomedical research. Quantitative measurements
%U https://www.openaccesspub.org/jpgr/article/71