%0 Journal Article
%T Radical-scavenging and Anti-oxidative Activities of TBN in Cell-free System and Murine H9c2 Cardiomyoblast Cells - Radical-scavenging and Anti-oxidative Activities of TBN in Cell-free System and Murine H9c2 Cardiomyoblast Cells - Open Access Pub
%A Gaoxiao Zhang
%A Longjun Zhu
%A Pei Yu
%A Yewei Sun
%A Yuqiang Wang
%A Zaijun Zhang
%J OAP | Home | Journal of Antioxidant Activity | Open Access Pub
%D 2018
%X Reactive oxygen species (ROS) and reactive nitrogen species are believed to be one of the most important culprits in the pathogenesis of cardio/cerebrovascular diseases. Intensive researches have been conducted to target free radicals as potential treatment for cardio/cerebrovascular diseases. The 2-(((1,1-dimethylethyl) oxidoimino)-methyl)-3,5,6-trimethylpyrazine (TBN), a novel nitrone derivative of tetramethylpyrazine, has been demonstrated to exhibit significant therapeutic effects in ischemic stroke and Parkinson＊s models due to its multiple functions, including calcium overload blockade and free radical-scavenging activity. In the present study, we found that TBN had significant radical trapping effect in cell-free assays. Additionally, TBN effectively blocked tert-butylhydroperoxide (t-BHP)-induced murine H9c2 cardiomyoblast cell death, suppressed H9c2 cell apoptosis and reversed the decrease in mitochondrial membrane potential. Furthermore, TBN markedly inhibited t-BHP-induced ROS generation and free radical NO and ONOO每.Taken together, these results suggest that TBN might be a potential candidate for the treatment of ischemic cardio/cerebrovascular diseases by targeting free radicals. DOI10.14302/issn.2471-2140.jaa-15-765 Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are a family of molecules that include molecular oxygen and its derivatives produced in all aerobic cells. They usually act as second messengers in cell signaling that are essential for various biological processes in normal cells1. It has been well documented that they regulate many signal transduction pathways by directly reacting with and/or modifying the structures of proteins, enzymes, transcription factors and genes to modulate their functions. Reactive oxygen species ROS, which are mainly generated in mitochondria, includes three types: superoxide anion radical (O2每), hydrogen peroxide (H2O2) and hydroxyl radical (OH), Approximately 1-3% of the oxygen taken up by the cell escapes from the mitochondrial electron transport chain, and is constitutively present in the form of O2每 2, 3, 4 O2 is subsequently converted to H2O2 by dismutation of O2每 or directly from the action of oxidase enzymes. Hydroxyl radical (OH), a highly reactive species that can be converted from H2O2 by Fenton reaction, will modify base pairs and cause strand breaks and result in DNA damage. RNS, often refers to nitric oxide (NO) and peroxynitrite (ONOO每). Nitric oxide radical (NO) can arise from L-arginine catalyzed by cytosolic or mitochondrial nitric oxide synthases (NOS) 5, while in
%U https://www.openaccesspub.org/jaa/article/210