%0 Journal Article
%T Identification of a Native Novel Oncolytic Immunoglobulin on Exfoliated Colon Epithelial Cells: A Bispecific Heterodimeric Chimera of IgA/IgG*
%A George P. Albaugh
%A Sudhir K. Dutta
%A Vasantha Iyengar
%A Samina Shami
%A Althaf Lohani
%A Eduardo Sainz
%A George Kessie
%A Prasanna Nair
%A Sara Lagerholm
%A Alka Kamra
%A J.-H. Joshua Chen
%A Shilpa Kalavapudi
%A Rakesh Vinayek
%A Robert Shores
%A Laila E. Phillips
%A Ram Nair
%A Padmanabhan P. Nair
%J Open Journal of Preventive Medicine
%P 126-150
%@ 2162-2485
%D 2020
%I Scientific Research Publishing
%R 10.4236/ojpm.2020.106010
%X Understanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (SCSR-010 Fecal Cell Isolation Kit, NonInvasive Technologies, Elkridge, MD), preserved at room temperature for up to one week, with viability of >85% and low levels of apoptosis (8% - 10%) exhibit two distinct cell size subpopulations, in the 2.5 ¦ÌM - 5.0 ¦ÌM and 5.0 ¦ÌM - 8.0 ¦ÌM range. In addition to IgA, about 60% of the cells expressed a novel heterodimeric IgA/IgG immunoglobulin that conferred a broad-spectrum cell mediated cytotoxicity against tumor cells. In a cohort of 58 subjects the exclusive absence of this immunoglobulin in two African-Americans was suggestive of a germline deletion. Serial cultures in stem cell medium retained the expression of this heterodimer. Since a majority of the cystic cells expressed the stem cell markers Lgr5 and Musashi-1 we termed these cells as gastrointestinal progenitor stem cells (GIP-C**). CXCR-4, the cytokine co-receptor for HIV was markedly expressed. These cells also expressed CD20, IgA, IgG, CD45, and COX-2. We assume that they originated from mature columnar epithelium by dedifferentiation. Our observations indicate that we have a robust noninvasive method to study mucosal pathophysiology and a direct method to create a database for applications in regenerative medicine.
%K Colon Epithelial Cells
%K CXCR-4
%K IgA/IgG Chimeric Immunoglobulin Heterodimer
%K COX-2
%K LGR-5
%K Musashi-1
%K Dedifferentiation
%K Cellular Engraftment
%K Oncoly-sis
%K Gastrointestinal Progenitor Stem Cells (GIP-C)
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=101213