%0 Journal Article
%T Development of an Analytical Method for Determination of Nitrate in Leafy Vegetables Using Ion Chromatography
%A Douglas Goncalves da Silva
%A Anaildes Lago de Carvalho
%A Evanildo de Jesus Ara¨²jo
%A Marcelo Eca Rocha
%A Bruno Oliveira Moreira
%A Dhavid Lucas Silva Santos
%J Open Access Library Journal
%V 7
%N 6
%P 1-8
%@ 2333-9721
%D 2020
%I Open Access Library
%R 10.4236/oalib.1106471
%X The presence of nitrate in food in general may indicate a threat to human health. Nitrate can be converted to nitrite, a toxic species, in saliva and in the gastrointestinal tract. This work describes a precise and sensitive method using ion chromatography to determine nitrate (NO-3) in hardwood vegetables. In the proposed method the extraction of the nitrate of the vegetal matrix was carried out in an aqueous medium at a temperature of 70¡ãC. The limit of detection (LOD) for the determined ion was 0.06 ¦Ìg¡¤L-1. The accuracy of the method was evaluated as the relative standard deviation (DPR) of the analytical signal area and the retention time, with values of 0.63% and 0.41%, respectively, for a solution of NO-3 5.0 mg¡¤L-1. The detector presented linearity between 0.22 and 10 mg¡¤L-1 with a correlation coefficient of 0.9999. The accuracy of the procedure was evaluated by addition/recovery test using real samples in which values greater than 95% were obtained. The validation of the proposed method was also performed by comparing the NO-3 concentrations obtained using the selective ion electrode method (EIS). The paired Student t test and the F test showed that both methods provide equivalent results concerning accuracy and precision.
%K Vegetables
%K Chemical Analysis
%K Nitrate
%K Ion Chromatography
%U http://www.oalib.com/paper/5435733