%0 Journal Article
%T 脐带间充质干细胞对高原脑水肿大鼠水通道蛋白-4的影响<br>The Effect of UC-MSCs on Aquaporins-4 Expression in High Altitude Cerebral Edema in Rats
%A 李娜
%A 宋星宜
%A 段敬豪
%A 孟慧鹏
%A 房伟
%A 张艳龙
%A 王克强
%A 董化江
%A 郝彬
%A 徐健
%A 杨术旺
%J Advances in Clinical Medicine
%P 670-675
%@ 2161-8720
%D 2020
%I Hans Publishing
%R 10.12677/ACM.2020.104105
%X <div style=\"text-align:justify;\">
	[目的]探讨间充质干细胞对高原脑水肿大鼠水通道蛋白4表达的影响。[方法]成功制作大鼠高原缺氧损伤模型8只，对照组及干细胞组各8只，测脑组织含水量，采用RT-PCR、免疫组织化学、Western Blot测定脑组织高原缺氧损伤24 h后水通道蛋白4 mRNA和蛋白表达变及间充质干细胞对其影响。[结果]高原缺氧损伤后，脑组织含水量增加，水肿明显，水通道蛋白4 mRNA和蛋白表达量上调(P &lt; 0.05)，应用间充质干细胞可有效抑制水通道蛋白4的表达。[结论]水通道蛋白4在高原缺氧脑水肿的形成过程中可能发挥了重要作用，应用间充质干细胞可有效抑制水通道蛋白4的表达，减轻高原脑水肿损伤。<br />
[Objective] To study the relationship between AQP4 and high altitude cerebral edema and effect of UC-MSCs. [Methods] The model of high altitude cerebral edema was established (N = 8). Brain edema was measured. The expression of AQP4 mRNA and protein were measured by RT-PCR, immunohistochemistry and Western Blot and to detect the effect of UC-MSCs in on Aquaporins-4 expression in high altitude cerebral edema in rats. [Results] The water content increased and the edema was evident. The expression of AQP4 mRNA and protein were up-regulated (P &lt; 0.05) and UC-MSCs could inhibit the expression of AQP4 in mRNA and protein. [Conclusions] The expression of AQP4 mRNA and protein were up-regulated after hypoxia. UC-MSCs could inhibit the expression of AQP4 in mRNA and protein in high altitude cerebral edema.
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%K 间充质干细胞，水通道蛋白4，大鼠，高原缺氧，脑水肿<br>UC-MSCs
%K Aquaporins-4
%K Rat
%K Plateau Hypoxia
%K Brain Edema
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=35395