%0 Journal Article
%T Development of <i>¦Á</i>-Amino Acid Detection with Peroxyoxalate Chemiluminescence by Using Water-Acetonitrile-Ethyl Acetate Mixed Solution
%A Hyo Kan
%A Ryo Mizutani
%A Kazuhiko Tsukagoshi
%J American Journal of Analytical Chemistry
%P 15-24
%@ 2156-8278
%D 2020
%I Scientific Research Publishing
%R 10.4236/ajac.2020.111002
%X Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. Eosin Y as a model fluorescence compound was dissolved with the ternary solutions of water (1.0 mM carbonate buffer, pH 9.0)-acetonitrile-ethyl acetate, water-rich of 15:3:2 volume ratio and organic solvent-rich of 3:8:4 volume ratio, to which bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide chemiluminescence reagent were added. The chemiluminescence observed with the ternary solutions, especially the organic solvent-rich solution, showed a larger signal than that observed with the water only solution or water-acetonitrile mixed solution. Chemiluminescence in the presence of twenty types of <i>¦Á</i>-amino acid was similarly examined by using the ternary organic solvent-rich solution. The chemiluminescence of three <i>¦Á</i>-amino acids with fluorescence properties was enhanced with the ternary solution. The data reported here may contribute to development of a new, sensitive peroxyoxalate chemiluminescence detection system.
%K Peroxyoxalate chemiluminescence
%K &lt
%K i&gt
%K ¦Á&lt
%K /i&gt
%K -Amino Acids
%K Water-Acetonitrile-Ethyl Acetate
%K Bis(2
%K 4
%K 6-Trichlorophenyl) Oxalate
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=98035