%0 Journal Article
%T Development and Characterization of New Microsatellite Markers for <i>Perilla frutescens</i> (L.) Britton
%A Kyu Jin Sa
%A Su Eun Lim
%A Ik-Young Choi
%A Kyong-Cheul Park
%A Ju Kyong Lee
%J American Journal of Plant Sciences
%P 1623-1630
%@ 2158-2750
%D 2019
%I Scientific Research Publishing
%R 10.4236/ajps.2019.109115
%X Based on RNA sequences using transcriptome analysis,
37 new simple sequence repeat (SSR) primer sets were developed for </span><i><span style=\"font-family:Verdana;\">Perilla</span></i><span style=\"font-family:Verdana;\"> species. These new SSR markers
were applied to analyze the genetic diversity among 15 accessions of </span><i><span style=\"font-family:Verdana;\">Perilla</span></i><span style=\"font-family:Verdana;\"> species. A total of 182 alleles
were confirmed in 37 loci, with an average of 4.9 alleles per locus and from 2
to 9 alleles per locus. The MAF (major allele frequency) per locus varied from
0.200 to 0.733, with an average of 0.463. The gene diversity (GD) ranged from
0.391 to 0.853, with an average of 0.670. The average polymorphic information
content (PIC) was 0.624, ranging from 0.315 to 0.838. The new SSR markers of </span><i><span style=\"font-family:Verdana;\">Perilla</span></i><span style=\"font-family:Verdana;\"> species reported in this study
may provide potential markers to analyze the genetic diversity and genetic
relationships of </span><i><span style=\"font-family:Verdana;\">Perilla</span></i><span style=\"font-family:Verdana;\"> species. In
addition, new </span><i><span style=\"font-family:Verdana;\">Perilla</span></i><span style=\"font-family:Verdana;\"> SSR markers
developed from transcriptome analysis can be useful for the identification of
cultivars, conservation of </span><i><span style=\"font-family:Verdana;\">Perilla</span></i><span style=\"font-family:Verdana;\"> germplasm resources, and genetic mapping and designating of important
genes/QTLs for future </span><i><span style=\"font-family:Verdana;\">Perilla</span></i><span style=\"font-family:Verdana;\"> crop breeding
programs.
%K &lt
%K i&gt
%K Perilla frutescens&lt
%K /i&gt
%K Oil Crop
%K Vegetable Crop
%K Genetic Diversity
%K Microsatellites
%K RNA-Seq
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=95256