In this study, the effects of different inducers on the accumulation of essential oil (1,8-eucalyptus) in suspension cells of Cinnamomum longepaniculatum were studied by adding different inducers, and the differences in the effects of different inducers on the yield and quality of essential oil in suspension cells of C. longepaniculatum were revealed. The results showed that under the same conditions, the addition of CaSO₄ 1.5 mg/L, Li2SO₄ 1.0 mg/L, SA15 mg/L and H₂O₂ 9 mmol/L in B5 medium could significantly promote the accumulation of essential oil, which was 60.94%, 54.69%, 36.72% and 35.16% higher than that of CK, reaching 0.0824 mg/L, 0.0792 mg/L, 0.0700 mg/L and 0.0692 mg/L, respectively. Through orthogonal test, it was found that 2.0 mg/L CaSO₄ combined with, H₂O₂ 9 mmol/L, SA 15 mg/L and Li2SO₄ 0.5 mg /L presented significant differences compared with other combinations (P < 0.05), and the yield reached 0.1768 mg/L, which increased by 245.31%. The experiment showed that the addition of inducer was an effective way to promote the accumulation of essential oil in suspension cells, and provided a reference for the manual regulation and strengthening of metabolism synthesis in this lifetime of suspension cells.