%0 Journal Article
%T Determination of Theobromine and Caffeine in <i>Theobroma cacao</i> Husk from Ethanolic Extract by GC-MS after CC Separation
%A Jos¨¦ Gonz¨¢lez
%A Max Monan
%A Julio Perez
%A Enrique G¨®mez
%A Damaris De La C. Salgado
%A Danae P¨¦rez
%J Open Access Library Journal
%V 6
%N 11
%P 1-9
%@ 2333-9721
%D 2019
%I Open Access Library
%R 10.4236/oalib.1105771
%X <div style="text-align:justify;">
	<span style="line-height:1.5;">After CC separation of 21 samples from Theobroma cacao Husk was analyzed by GC-MS, from ethanolic extract at 90% was recovered a solid residue of 1 g after several days on the table of the Lab. This main residue (300 mg) was analyzed after successive separations with mixtures of CHCl3: MeOH (7:3) and CHCl3: MeOH (5:5) in CC using S¨ªlica Gel G 60-120 Mesh. All samples were analyzed by TLC on silica gel with fluorescent indicator 254 nm on aluminum cards (layer thickness 0.2 mm) (10 ¡Á 20 cm) using CHCl3: MeOH: n-propanol: water (5:6:1:4) as eluent (v/v/v). The presence of Caffeine (CF) and Theobromine (TB) was confirmed after several analyses of the samples by their characteristic fragmentation pattern but nevertheless was found the presence of Theophylline.</span>
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%K <i>Theobroma cacao</i>
%K Caffeine
%K Theobromine
%K GC-MS
%K CC
%K TLC
%U http://www.oalib.com/paper/5421795