%0 Journal Article %T 零换水条件下枯草芽胞杆菌和糖蜜对水质和罗非鱼生长的影响<br>Effects of Bacillus subtilis and molasses on water quality and growth of tilapia under zero-water exchange %A 唐汇娟 %A 张轩豪 %A 孔重敏 %A 郝乐 %A 李博星 %A 余祥勇 %A 刘丽 %J 华中农业大学学报 %D 2018 %X 在零换水条件下采用全池泼洒枯草芽胞杆菌（Bacillus subtilis）菌液并添加甘蔗糖蜜作为碳源，研究其对罗非鱼(Oreochromis niloticus)生长性能和水质的影响。研究共设置4组：空白组、单独添加糖蜜组（C组）、单独添加枯草芽胞杆菌组（Y组）、同时添加糖蜜和枯草芽胞杆菌组（C+Y组）。试验持续6周，期间水温26~31℃，pH为6.5~7.5。研究结果显示，糖蜜和枯草芽胞杆菌的添加显著降低了养殖水体中的溶氧含量，糖蜜的添加提高了总氨的含量（P＜0.05），相对于空白组，枯草芽胞杆菌和蜜糖的联合使用能显著降低水体亚硝酸盐氮、硝酸盐氮和反应性磷的含量。在整个养殖期间，空白组和实验组浮游植物丰度都比较低(104~106个/L)。但相对而言，3个处理组浮游植物丰度在养殖后期要远远高于空白组，以鞭毛藻（隐藻Cryptomonas和甲藻Gymnodinium）和硅藻（Bacillariophyta）为主。枯草芽胞杆菌和碳源的添加在一定程度提高了罗非鱼的存活率、生长率以及饲料转化效率，但并未达到显著水平。<br>This study was carried out to evaluate the effects of Bacillus subtilis and molasses on water quality and growth of tilapia (Oreochromis niloticus) under zero-water exchange.Four experimental groups including treatments with molasses alone (C),Bacillus subtilis alone (Y), molasses and Bacillus subtilis (C+Y) and the negative blank were designed.During the experiment,water temperature ranged from 26 to 31℃ and pH ranged from 6.5 to 7.5.The mean dissolved oxygen was significantly higher in the blank than the 3 treatments (P＜0.05).The groups with utilization of molasses alone and together with B. subtilis had significantly higher total ammonia contents (P＜0.05).Combined utilization of molasses and B. subtilis showed the lowest level of nitrite,nitrate nitrogen and soluble reactive phosphorus.Overall,phytoplankton density in all groups was rather low (104-106 cells/L),but the three treatments showed higher phytoplankton density than the blank during the late period of the experiment.Survival rate,growth rate of tilapia and feed efficiency were higher in the 3 treatments than that in the blank,but the difference is not significant (P＞0.05). %K 罗非鱼 枯草芽胞杆菌 糖蜜 水质 生长< %K br> %K Oreochromis niloticus Bacillus subtilis molasses water quality growth %U http://hnxbl.cnjournals.net/hznydxzr/ch/reader/view_abstract.aspx?file_no=20180312&flag=1