%0 Journal Article %T 沈阳地区油松枝枯病病原菌的鉴定<br>Pathogen Identification of the Shoot Blight of Chinese Pine in Shenyang %A 王月 %A 高国平 %A 张瑶琪 %A 周阳 %A 王允 %A 祁金玉 %J 西北林学院学报 %D 2015 %R doi:10.3969/j.issn.1001-7461.2015.03.24 %X 采用传统形态分类学和现代分子生物学(DNA测序)相结合的方法,对沈阳地区近些年发生的油松枯枝病病原菌进行了分类鉴定研究,对筛选的2种真菌的子实体进行了接种试验。结果表明,在沈阳地区油松发病枯死枝上分离到的6个菌株(A1、A2、A3、A4、B1与B2)为2种菌,其中,菌种A为铁锈薄盘菌(Cenangium ferruginosum Fr. ex Fr.),菌种B为松丛赤壳菌(Nectria cucurbitula Sacc.)。2种真菌对油松健康小枝条均有侵染致病性,并分别获得有性子实体,证明了2种真菌为引起油松枯枝病的病原菌。<br>Traditional morphological taxonomy and modern molecular biology(DNA sequencing) were combined to classify and identify pathogenic fungi of Chinese pine shoot blight in Shenyang. Six strains(No. A1-A4,B1-B2) were isolated on dead branches of Pinus tabulaeformis, belonging to two fungus species. Strain A was Cenangium ferruginosum Fr.ex.Fr, strain B was Nectria cucurbitula Sacc. Two fungus species all presented pathogenicity in small healthy branches of P. tabulaeformis. The sexual fruiting bodies were obtained, indicating 2 species of fungi belonged to pathogenic fungi for P. tabulaeformis branches disease %K 油松 %K 枯枝病 %K 病原菌鉴定 %K 接种试验< %K br> %K Pinus tabulaeformis %K shoot blight %K identification of pathogenic fungi %K inoculation test %U http://xblxb.paperopen.com/oa/darticle.aspx?type=view&id=201503024