%0 Journal Article
%T 羊草 14-3-3基因的克隆及序列分析<br>Isolation and Expression Analysis of 14-3-3 Gene from Leymus chinensis
%A 赵
%A 英
%A 刘晶莹
%A 杨梦丹
%A 汪自庆
%A 麻鹏达
%J 西北农业学报
%D 2018
%R 10.7606/j.issn.1004-1389.2018.02.015
%X 通过羊草（ Leymus chinensis)EST(Expressed sequence tags,EST)序列和RACE(Rapid-amplification of cDNA ends)技术，获得羊草14-3-3蛋白的全长基因为1 048 bp，具有792 bp开放读码框（Open reading frame，ORF）、81 bp的5′非编码区(Untranslated regions,UTR)和175 bp的3′ UTR，编码含有263个氨基酸的蛋白质。通过序列比对，羊草14-3-3蛋白与其他物种14-3-3蛋白相似度达到80%～99%。通过RT-PCR（Reverse transcription-PCR）分析表明羊草14-3-3基因在叶片和根部均有表达，且叶片的表达量明显高于根部，而经过NaCl、Na2CO3和PEG的胁迫处理，羊草叶片14-3-3基因表达量无明显变化。本研究有助于了解14-3-3蛋白家族基因的功能，为进一步应用该基因进行植物的耐盐性遗传改良奠定基础。<br>The 14-3-3 protein is a family of regulatory proteins encoded by a gene.Although genome-wide analysis of this family has been carried out in some plant species, little is known about 14-3-3 genes in Leymus chinensis.In this study, the full-length gene of Leymus chinensis14-3-3 protein was obtained by EST and RACE.The gene was 1 048 bp in length with an ORF of 792 bp, a 5′-untranlstaed region(UTR) of 81 bp and a 3′UTR of 175 bp, which encoded a predicted polypeptide of 263 amino acids.By sequence alignment, the similarity of 14-3-3 protein of Leymus chinensis and other species 14-3-3 protein was 80%-99%.The results of reverse transcription-PCR showed that Leymus chinensis 14-3-3 protein was expressed in leaves and roots, and the contents of leaves were higher than those of roots.Tracing with NaCl, Na2CO3 and PEG 6000,the content of Leymus chinensis 14-3-3 protein did not change significantly.This study is helpful to understand the function of 14-3-3 protein family gene, which can lay the foundation for further application of this gene for salt tolerance genetic improvement inplant.
%K 14-3-3蛋白 羊草 RACE RT-PCR&lt
%K br&gt
%K 14-3-3 protein Leymus chinensis RACE RT-PCR
%U http://xbnyxb.alljournals.cn/ch/reader/view_abstract.aspx?file_no=20180215&flag=1