%0 Journal Article
%T DNA条形码技术在几种苹果园鳞翅目害虫鉴定中的应用<br>Application of DNA Barcoding Technology to Identification of Some Lepidoptera Pests in Apple Orchards
%A 李晓叶
%A 钱
%A 路
%A 陈茂华
%J 西北农业学报
%D 2015
%R 10.7606/j.issn.1004-1389.2015.05.023
%X 为探讨DNA条形码技术在苹果园鳞翅目昆虫鉴定中的适用性，采用DNA条形码通用引物，扩增并分析采自陕西7个苹果园的鳞翅目昆虫的线粒体细胞色素氧化酶Ⅰ基因（COⅠ）片段，基于扩增的COⅠ序列计算种间和种内遗传距离，并构建NJ树（Neighbor-Joining tree）和ME树(Minimum-Evolution tree) 。经通用的DNA条形码技术鉴定显示，采集的鳞翅目昆虫隶属于7个属的9种昆虫；基于p-distance模型的种内和种间遗传距离分析显示，种间遗传距离为8.4%～69.4%，种内遗传距离为0～0.6%，种内最大遗传距离与种间最小遗传距离重叠；同时，基于种间和种内遗传距离构建NJ树和ME树均显示，同种昆虫在系统树上都各自在同一进化支；因此，基于DNA条形码的鉴定方法可区分这些苹果园的不同鳞翅目害虫。<br>In order to test the feasibility of DNA barcoding based on the mitochondrial cytochrome oxidase subunit Ⅰ gene (COⅠ) on identification of Lepidoptera pests from apple orchards, we collected samples from seven apple orchards in Shaanxi province, and amplified the COⅠ fragments of each sample. The obtained COⅠ sequences were compared and BLAST in GenBank and BOLD database. Genetic distance were estimated using the p-distance model in MEGA version 5.0. Neighbor-joining (NJ) and Minimum-evolution (ME) tree based on genetic distance were constructed. Bootstrap values were calculated with 1 000 replicates.The results showed that the specimens belonged to 9 species of 7 genera. The intraspecific genetic distance was 8.4%-69.4%, whereas the interspecific genetic distance was 0-0.6%. There was no overlapping between the intraspecific genetic distance and the interspecific genetic distance. The COⅠ haplotypes of each of the species were included in a respective branch in both the NJ tree and ME tree. Our results suggests that DNA barcoding can be used as an effective method to identify Lepidoptera species in apple orchards.
%K 鳞翅目害虫 DNA条形码 COⅠ基因&lt
%K br&gt
%K Lepidopteron pest DNA barcoding COⅠ gene
%U http://xbnyxb.alljournals.cn/ch/reader/view_abstract.aspx?file_no=20150523&flag=1