%0 Journal Article
%T 影响甘蓝小孢子DH植株生长的几个因素<br>Influence of Several Factors on Growth of DH Plants Regenerated from Microspores in Cabbage
%A 刘
%A 争
%A 张恩慧
%A 程永安
%A 许忠民
%A 王改改
%J 西北农业学报
%D 2016
%R 10.7606/j.issn.1004-1389.2016.04.017
%X 旨在筛选甘蓝小孢子DH植株健壮生长的最适培养基和光照强度。为缩短小孢子DH植株培养时间并为获得健壮植株创造条件，从而加快甘蓝DH系育种进程。以甘蓝‘秦甘50’为材料进行游离小孢子培养至诱导分化出不定芽，转接到NAA质量浓度不同的2种培养基上（1/2MS+0、0.1、0.2、0.3 mg/L NAA和MS+0.1 mg/L NAA），不同光照强度［40、60、80、100 μmol/(m ·s)］及添加不同质量浓度NH4NO3（0、0.4、0.8、1.2 g/L）培养，研究DH植株的生长势，获得甘蓝小孢子DH植株最适培养基和培养条件。结果表明，在NAA质量浓度不同的培养基中，1/2MS +0.1 mg/L NAA培养基中小孢子DH植株生长速度最显著，生根快、植株长势强；在80 μmol/(m ·s)光照强度下培养小孢子DH植株净生长高度显著，10.3 d再生出根系,根质量较好，叶片大而厚；MS培养基中添加质量浓度为0.4 g/L的NH4NO3，小孢子DH植株生长健壮，35 d内植株净生长高度最大为56.25 mm。以上结果说明，甘蓝小孢子DH植株快速健壮生长的适宜培养基为1/2MS+0.1 mg/L NAA+8 g/L琼脂+30 g/L蔗糖；在80 μmol/(m ·s)光照强度下或MS培养基中添加质量浓度为0.4 g/L的NH4NO3均能够促进甘蓝小孢子DH植株根茎叶生长旺盛。<br>Aims to screen the most appropriate medium and illumination for DH plants regenerated from microspore in cabbage and to shorten culture time of obtaining robust plants, which will accelerate the breeding process of cabbage double haploid. Microspores of ‘Qin-gan 50’ cabbage was cultured until emergence of adventitious buds which then transferred to two kinds of mediums (1/2MS+0,0.1,0.2,0.3 mg/L NAA and MS+0.1 mg/L NAA), adding different amount of NH4NO3(0,0.4,0.8,1.2 g/L) in MS medium and cultured at different illumination ［40,60,80,100 μmol/(m ·s)］ . Then the growth vigor of the DH plants were studied and the most appropriate medium and culture conditions could be gotten. The DH plants grown in 1/2MS +0.1 mg/L NAA medium had the fastest growth rate and strong growth vigor, rooting fast;cultured in the illumination of 80 μmol/(m ·s),the DH plants had the most remarkable growth speed, better roots and leaves, which rooted in 10.3 days;the DH plants in MS medium with added 0.4 g/L NH4NO3 had the maximum value 56.25 mm of net growth height in 35 days. In conclusion, the appropriate medium for the DH plants regenerated from microspore in cabbage is 1/2MS+0.1 mg/L NAA+8 g/L Agar+30 g/L Sugar;cultured in the illumination of 80 μmol/(m ·s) or transferred into the MS medium with added 0.4 g/L NH4NO3, the DH plants grown well with robust roots, stems and leaves.
%K 甘蓝 DH植株 小孢子再生 培养基 光照强度 NH4NO3&lt
%K br&gt
%K Cabbage DH plants Regeneration from microspores Medium Illumination NH4NO3
%U http://xbnyxb.alljournals.cn/ch/reader/view_abstract.aspx?file_no=20160417&flag=1