%0 Journal Article
%T 花绒寄甲Caspase-1基因在不同虫态的表达<br>Dastarcus helophoroides (Coleoptera:Bothrideridae)Caspase-1 Gene Expression in Different Stages
%A 屈
%A 赟
%A 赵乐乐
%A 高盼盼
%A 任春艳
%A 翟俊伟
%A 李孟楼
%J 西北农业学报
%D 2015
%R 10.7606/j.issn.1004-1389.2015.12.025
%X 为明确花绒寄甲Caspase-1基因及其蛋白酶活性在不同虫态中的表达特性，采用RT-PCR和Caspase酶活测定方法进行研究。结果表明：Caspase-1酶活性变化趋势与其基因表达趋势基本一致，Caspase-1基因及其蛋白酶在5龄幼虫期和蛹期有较高的活性，且其活性高于所有成虫期；成虫期随存活时间的延长，Caspase-1酶活性总体呈现增长趋势。Caspase-1及其酶活在不同虫态中表达规律的揭示，为研究Caspase-1基因与花绒寄甲生理代谢和寿命关系提供依据。<br>We determinned the gene expression level of Dastarcus helophoroides (Fairmaire),through the method of real-time PCR;the protease activity through the determination of Caspase enzyme activity. The studies showed that the enzyme activity changed as its gene expression. Caspase-1 and its protease had higher activity in 5 th instar and pupal stage.Caspase-1 enzyme activity showed a trend of growth as the extensin of survival time of adults. This study laid foundation for the futher study in physiological metabolism and aging mechanism.
%K 花绒寄甲 Caspase-1基因 Caspase酶 表达量&lt
%K br&gt
%K Dastarcus helophoroides Caspase-1 Caspase enzyme Expression value
%U http://xbnyxb.alljournals.cn/ch/reader/view_abstract.aspx?file_no=20151225&flag=1