%0 Journal Article
%T 基于真空渗入法的转基因大白菜植株的获得<br>Obtaining Transgenic Plants of Chinese Cabbage Based on Vacuum Infiltration
%A 赵
%A 静
%A 吴俊清
%A 李庆飞
%A 张
%A 静
%A 张鲁刚
%J 西北农业学报
%D 2017
%R 10.7606/j.issn.1004-1389.2017.02.013
%X 采用真空渗入法，将萝卜 VPE1基因的干扰载体pRNAi- RsVPE1转入大白菜中，共收获2 414粒种子，在质量浓度为30 mg/L的卡那霉素筛选下获得抗性株297株，其中9株经PCR检测呈阳性，转化率为0.37%。Real-time PCR检测结果表明，pRNAi- RsVPE1的转入导致转基因大白菜叶片中同源的BraVPE在RNA水平上的表达量下降，验证了转基因植株的可靠性，为进一步深入研究BraVPE基因功能奠定了基础。<br>The vector pRNAi- RsVPE1 was transformed into Chinese cabbage by vacuum infiltration method and total 2 414 seeds were obtained. The kanamycin concentration 30 mg/L was determined to be the most suitable for selecting transgenic plants. 297 transgenic plants resistant to kanamycin were obtained,of which 9 positive transgenic plants were verified by PCR detection,and the transgenic rate was 0.37%. RT-PCR detection showed that the expression level of BraVPE in transgenic plants decreased significantly,reliability of transgenic plants was verified. Therefore ,the above results laid a foundation for further study in BraVPE gene functions.
%K 真空渗入法 大白菜 BraVPE转基因 RNAi载体&lt
%K br&gt
%K Vacuum infiltration Chinese cabbage BraVPE Genetic transformation RNAi vector
%U http://xbnyxb.alljournals.cn/ch/reader/view_abstract.aspx?file_no=20170213&flag=1