%0 Journal Article
%T 小麦-簇毛麦易位系中贮藏蛋白的鉴定及分析<br>Identification and Analysis of Storage Protein in Chinese Spring (CS)-Dasypyrum villosa Translocation Lines
%A 刘皋芃
%A 高
%A 翔
%A 杨明明
%A 赵万春
%A 董
%A 剑
%A 石引刚
%A 陈良国
%J 西北农业学报
%D 2016
%R 10.7606/j.issn.1004-1389.2016.09.005
%X 为探讨簇毛麦染色体对小麦品质的影响，利用A-PAGE、SDS-PAGE和高效毛细管电泳（High-performance capillary electrophoresis，HPCE）技术对12个易位系及7个附加系的贮藏蛋白进行鉴定。将簇毛麦的一条ω-醇溶蛋白基因定位于1VL。结果表明：易位系T1DL·1VS可用来改良小麦品质；定位于簇毛麦1VS染色体上的高分子谷蛋白在小麦中表达不稳定；其他材料醇溶蛋白组成发生改变较小，可能对面筋相关品质影响不大，在育种工作中不会引起面筋相关品质下降；易位系材料中的小麦醇溶蛋白的质量分数有所增加。<br>For studying the effects of Dasypyrum villosa chromsomes on grain processing quality of Chinese Spring(CS),A-PAGE SDS-PAGE and HPCE were used to analyze to identify the seed storage protein of 12 translocation lines and 7 addition lines in this paper. One of the ω-Gliadin was located in the 1VL，α-Gliadin located in the 1VS,4VS and 6VL. The results showed that the translocation lines T1DL·1VS could improve wheat quality. Expression level of the HMW-GS gene Glu-V1 of D.villosum which located on 1VS was low.The gliadin contents of other translocation lines changed a little, and it maybe had little effect on gluten quality, so it cannot decrease related gluten quality in wheat breeding, the gliadin content of wheat in translocation lines increased.
%K 簇毛麦 易位系 贮藏蛋白 小麦品质&lt
%K br&gt
%K D.villosum Translocation lines Storage protein Wheat quality
%U http://xbnyxb.alljournals.cn/ch/reader/view_abstract.aspx?file_no=20160905&flag=1