%0 Journal Article
%T 长期乙醇暴露对大鼠肺组织中TREM-1/2通路表达的影响*<br>Effect of long-term alcohol exposure on TREM-1/2 pathway expression in rat lung tissue
%A 张伟宏
%A 徐高磊
%A 易诗琪
%A 张译铮
%A 沈建明
%A 杨静尘
%A 杨东斌
%J 郑州大学学报（医学版）
%D 2017
%R 10.13705/j.issn.1671-6825.2017.06.008
%X 目的:探讨长期乙醇暴露对大鼠肺组织中TREM-1/2通路表达的影响。方法:将40只SD大鼠随机分为2组。乙醇组按2.5 mL/(kg？d)的剂量灌胃体积分数20%的乙醇; 对照组灌胃等量清水。4周后,运用免疫组化染色及Western blot技术检测大鼠肺组织中TREM-1、TREM-2及信号衔接蛋白DAP-12的表达。结果:4周后,对照组大行动敏捷,食欲良好,无腹泻,体重增加明显; 与对照组比较,乙醇组大鼠体重无明显增加,出现腹泻症状,大鼠肺组织中TREM-1表达下调,TREM-2和DAP-12表达上调(P<0.05)。结论:长期乙醇暴露可能破坏大鼠肺巨噬细胞TREM-1/2通路的平衡,导致大鼠免疫力低下。<br>Aim: To investigate the effect of long-term alcohol exposure on the expressions of TREM-1,TREM-2 and DAP-12 in rat lung tissue.Methods: Forty SD rats were allocated into 2 groups randomly. The rats in alcohol group were lavagely given alcohol(volume fraction of 20%)at dose of 2.5 mL/(kg？d), and those in control group were given water. After four weeks, immunohistochemical staining and Western blot technique were used to detect the expressions of TREM-1,TREM-2 and DAP-12 in lung tissue.Results: After 4 weeks, the control rats had agile action, good appetite, no diarrhea, and gained weight obviously. Compared with control group, the rats in alcohol group had no significant weight gain, and showed diarrhea symptoms, TREM-1 expression in rat lung tissue decreased, and TREM-2 and DAP-12 expressions increased(P<0.05).Conclusion: Long-term alcohol exposure may destroy the balance of TREM-1/2 pathway, thereby cause low immunity
%K 肺
%K 乙醇
%K TREM-1
%K TREM-2&lt
%K br&gt
%K lung
%K alcohol
%K TREM-1
%K TREM-2
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201706008