%0 Journal Article
%T 低氧对滋养细胞HIF-1α、BNIP3表达及自噬和侵袭能力的影响<br>Effects of hypoxia on expressions of HIF-1α,BNIP3 and autophagy, invasion ability in trophoblasts
%A 闫广伟
%A 丁燕子
%A 邢金芳
%A 代延朋
%A 杜红梅
%A 袁恩武
%J 郑州大学学报（医学版）
%D 2018
%R 10.13705/j.issn.1671-6825.2017.07.043
%X 目的:探讨低氧对滋养细胞HIF-1α、BNIP3表达及自噬和侵袭能力的影响。方法:体外培养滋养细胞HTR-8/SVneo,分为常氧组、缺氧组和3-MA(自噬抑制剂)+缺氧组。24 h后,分别采用Real-time PCR和Western blot检测常氧组与缺氧组滋养细胞HIF-1α、BNIP3 mRNA和蛋白的表达水平; 采用Western blot检测3组滋养细胞自噬标志蛋白Beclin 1和LC3蛋白的表达水平,并用Transwell侵袭实验测定细胞的侵袭能力。结果:与常氧组相比,缺氧组BNIP3 mRNA及HIF-1α、BNIP3蛋白的表达水平升高(P<0.05),但HIF-1α mRNA的表达水平无明显变化(P>0.05)。与常氧组相比,缺氧组Beclin 1、LC3Ⅱ/Ⅰ蛋白的表达水平升高,侵袭细胞数增加; 与缺氧组相比,3-MA+缺氧组Beclin 1和LC3Ⅱ/Ⅰ蛋白表达水平降低,侵袭细胞数减少(P<0.05)。结论:低氧环境可能通过HIF-1α/BNIP3途径诱导滋养细胞自噬水平增强,并可以增强其侵袭能力; 低氧环境下抑制自噬后滋养细胞侵袭性减弱。<br>Aim: To investigate the effects of hypoxia on the expressions of HIF-1α and BNIP3,and assess the autophagy and invasion ability of trophoblasts.Methods: First,HTR-8/SVneo cells were divided into control group,hypoxia group,and autophagy inhibitor 3-MA plus hypoxia group(3-MA+ hypoxia group). After 24 h, Real-time PCR and Western blot were utilized to measure the expression levels of HIF-1α and BNIP3 mRNA and protein in the first 2 groups. Then the expression levels of Beclin1 and LC3 protein as well as the invasion activity of the cells were detected by Western blot and Transwell Matrigel assay.Results: Compared with control group,the expression level of HIF-1α mRNA in hypoxia group had no significant difference(P>0.05); however,the expression levels of BNIP3 mRNA and protein and that of HIF-1α protein in hypoxia group were increased apparently(P<0.05). Compared with control group, the expression levels of Beclin 1 and LC3Ⅱ/Ⅰprotein and the invasion ability of trophoblasts increased(P<0.05); compared with hypoxia group,the expression levels of Beclin 1 and LC3Ⅱ/Ⅰ protein decreased,and the invasion ability was reduced in 3-MA+hypoxia group(P<0.05).Conclusion: Hypoxia could induce autophagy and enhance the invasion ability of trophoblasts via the HIF-1α/BNIP3 pathway; the invasion ability of trophoblasts under hypoxia is decreased in the presence of 3-MA
%K 缺氧
%K 滋养细胞
%K 自噬
%K HIF-1α&lt
%K br&gt
%K hypoxia
%K trophoblast
%K autophagy
%K HIF-1α
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201802014