%0 Journal Article
%T 柯萨奇病毒B3诱导的病毒性心肌炎C57BL/6J小鼠模型的建立<br>Establishment of C57BL/6J mouse myocarditis model induced by coxsackievirus B3
%A 赵小建
%A 张洁钰
%A 杨
%A 帆
%A 王
%A 丽
%A 张真真
%A 唐
%A 琳
%A 赵洛沙
%J 郑州大学学报（医学版）
%D 2016
%X 目的:建立由柯萨奇病毒B3(CVB3)Nancy株诱导的病毒性心肌炎(VMC)C57BL/6J小鼠模型。方法:4周龄、6周龄C57BL/6J小鼠各50只,每个年龄组随机选取15只作为对照(腹腔注射PBS),剩余35只腹腔接种含CVB3 Nancy株的病毒悬液(1×105 PFU/只),于感染后第14天取心脏,行 HE染色和CD3、CD45免疫组化染色,进行病理学评价,检测血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平,并做心肌组织中病毒滴度检测,采用Western blot法检测磷酸化AKT、IkBα、GSK3β的表达。结果:4周龄小鼠造模过程中有19只(54.3%)死亡; 与对照小鼠比较,模型小鼠心肌呈现弥漫性炎性细胞浸润和片状心肌细胞变性坏死, CD3、CD45阳性细胞弥漫浸润于心肌间质,血清IL-1β、TNF-α水平明显升高(P<0.05),心肌组织中磷酸化AKT、IkBα、GSK3β表达增高(P<0.05)。6周龄小鼠造模过程中有3只(8.6%)死亡,模型小鼠心肌组织中炎症反应轻于4周龄模型小鼠(P<0.05)。结论:成功建立了 VMC 的C57BL/6J小鼠模型, 4周龄C57BL/6J小鼠更适用于模型的建立。<br>Aim: To establish a C57BL/6J mouse model of myocarditis induced by coxsackievirus B3(CVB3).Methods: A total of 50 male mice with 4-week-age or 6-week-age were divided into two groups: the control(n=15)were injected via intraperiton with PBS, and the model group(n=35)were injected via intraperiton with 1×105 PFU CVB3. After 14 d, mice hearts were harvested for HE staining and CD3,CD45 immunohistochemistry staining,the serum levels of IL-1β,TNF-α were assayed by ELISA, the expressions of p-AKT,p-IkBα and p-GSK3β were detected by Western blot.Results: 4-week-age model group came out with a higher mortality(19/35,54.3%), HE staining showed that a great deal of inflammatory cells concentrated in the adjacent area around the myocardiocytes,which was consistent with the finding that many CD3,CD45 positive macrophages gathered in the necrosis area,TNF-α and IL-6 level were increased(P<0.05),p-AKT,p-IκBα and p-GSK3β protein expression increased(P<0.05). While the 6-week-age model group showed a lower mortality(3/35,8.6%),and the immune response to CVB3 infection was milder than 4-week-age model group(P<0.05).Conclusion: A C57BL/6J mouse model of myocarditis induced by CVB3 is successfully established,and 4-week-age mice are recommended for this model
%K 柯萨奇病毒B3
%K 病毒性心肌炎
%K C57BL/6J小鼠
%K 动物模型&lt
%K br&gt
%K coxsackievirus B3
%K viral myocarditis
%K C57BL/6J mouse
%K animal model
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201602019