%0 Journal Article
%T CNRIP1启动子区去甲基化对结肠癌细胞糖酵解及增殖、迁移活性的影响<br>Effects of CNRIP1 promoter demethylation on glycolysis, proliferation and migration activity of colon cancer cells
%A 冯馨园
%A 张
%A 婷
%A 崔
%A 戈
%A 王旗春
%A 钱
%A 乙
%A 蔡
%A 莹
%A 仰舒静
%A 张梦荧
%A 姚嘉诚
%J 郑州大学学报（医学版）
%D 2018
%R 10.13705/j.issn.1671-6825.2017.04.074
%X 目的:探讨CNRIP1启动子区去甲基化对结肠癌细胞糖酵解途径、细胞增殖及迁移活性的影响。方法:采用5-氮杂胞苷(5-azaC)降低结肠癌细胞SW620 CNRIP1启动子区甲基化水平,以未干预细胞作对照。用亚硫酸盐测序法检测细胞CNRIP1启动子区甲基化水平; 分别采用qRT-PCR、Western blot法检测细胞中CNRIP1、糖酵解途径关键酶HK2、PKM2、LDH-A mRNA及蛋白表达水平,生化法检测细胞培养液中乳酸水平; 分别采用CCK-8比色法、Transwell法检测细胞增殖及迁移能力。结果:与未干预组细胞相比,5-azaC干预组细胞CNRIP1启动子区甲基化水平降低,CNRIP1 mRNA及蛋白表达升高,HK2、PKM2、LDH-A mRNA和蛋白表达水平及细胞培养液中乳酸水平均降低,细胞增殖及迁移能力均减弱(P均<0.05)。结论:CNRIP1去甲基化可抑制糖酵解途径,从而降低结肠癌细胞的增殖及迁移能力。<br>Aim: To investigate the effects of cannabinoid receptor interacting protein 1(CNRIP1)promoter demethylation on glycolysis, proliferation, and migration potential of colon cancer cells.Methods: CNRIP1 promoter methylation in colon cancer cells SW620 was suppressed via treatment with 5-azacytidine(5-azaC), and the CNRIP1 promoter methylation level was determined by bisulfite sequencing PCR; the mRNA and protein expression levels of CNRIP1 as well as key enzymes(HK2, PKM2, LDH-A)of glycolysis pathway were determined by qRT-PCR and Western blot, respectively; lactic acid level in culture medium was determined with biochemistry kit. The proliferative and migration abilities of colon cancer cells were determined with CCK-8 and Transwell cell assays, respectively.Results: Compared with untreated group, CNRIP1 promoter methylation level was decreased significantly in SW620 cells treated with 5-azaC, mRNA and protein expressions of CNRIP1 were increased significantly, mRNA and protein expressions of HK2, PKM2 and LDH-A were depressed significantly, and the proliferative and migration abilities were depressed significantly(P<0.05).Conclusion: CNRIP1 promoter demethylation could depress the glycolysis pathway, and reduce the proliferative and migration abilities in colon cancer cells
%K 结肠癌
%K 大麻素受体互作蛋白1
%K 去甲基化
%K 糖酵解
%K 增殖
%K 迁移&lt
%K br&gt
%K colon cancer
%K cannabinoid receptor interacting protein 1
%K demethylation
%K glycolysis
%K proliferation
%K migration
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201801018