%0 Journal Article
%T miR-101对结直肠癌细胞增殖、凋亡及KIF14蛋白表达的影响<br>Effect of miR-101 on proliferation,apoptosis and KIF14 expression of colorectal cancer cells
%A 刘继攀
%A 孙
%A 伟
%A 赵学荣
%A 李炳茂
%A 王成君
%J 郑州大学学报（医学版）
%D 2018
%R 10.13705/j.issn.1671-6825.2018.11.003
%X 目的:研究miR-101对结直肠癌细胞增殖、凋亡及驱动蛋白家族蛋白14(KIF14)表达的影响。方法:利用靶基因预测库预测miR-101与KIF14的靶向关系,并采用双荧光素酶报告实验鉴定。采用实时荧光定量PCR和Western blot法测定结直肠癌组织和细胞中miR-101和KIF14蛋白的表达。在结直肠癌HT29细胞中转染miR-101 模拟物,MTT法测定细胞增殖情况,Annexin V-FITC/PI双染法检测细胞凋亡率,Western blot法测定KIF14蛋白的表达。结果:双荧光素酶报告实验结果显示,miR-101与KIF14存在靶向关系。结直肠癌组织和细胞中miR-101表达下调,KIF14表达上调(P<0.05)。miR-101 模拟物可以明显抑制HT29细胞的增殖能力并促进其凋亡,同时下调KIF14蛋白表达水平(P<0.05)。结论:miR-101可靶向作用于KIF14而影响结直肠癌细胞的增殖和凋亡。<br>Aim: To study the effect of miR-101 on proliferation,apoptosis and KIF14 expression of colorectal cancer cells.Methods: Target gene prediction library was used to predict the targeting relationship between miR-101 and KIF14, and double luciferase reporter assay was used to identify the relationship.The expressions of miR-101 and KIF14 in colorectal cancer tissue and colorectal cancer cells were detected by qRT-PCR and Western blot.miR-101 mimics was transfected into HT29 cells,and then cell proliferation was measured by MTT,apoptosis was detected by Annexin V-FITC/PI staining,Western blot was used to detect the KIF14 protein expression.Results: The double luciferase reporter assay showed that there was a target relationship between miR-101 and KIF14.miR-101 was downregulated and KIF14 was upregulated in colorectal cancer tissue and colorectal cancer cells(P<0.05).miR-101 mimics significantly inhibited the proliferation and KIF14 protein expression,and promote apoptosis in HT29 cells(P<0.05).Conclusion: miR-101 could inhibit proliferation and induce apoptosis of colorectal cancer cells by targeting KIF14
%K miR-101
%K 结直肠癌
%K KIF14
%K 凋亡&lt
%K br&gt
%K miR-101
%K colorectal cancer
%K KIF14
%K apoptosis
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201806019