%0 Journal Article
%T HPV-16 L1甲基化和HPV E6/E7 mRNA检测宫颈病变的临床价值<br>Clinical value of HPV？？16 L1 methylation and HPV E6/E7 mRNA testing in detection of cervical lesions
%A 李肖甫△
%A 邱翠
%A 智艳芳
%A 李雅
%A 荣守华
%A 樊婷婷
%J 郑州大学学报（医学版）
%D 2016
%X 目的：探讨HPV？？16 L1甲基化程度、HPV E6/E7 mRNA表达水平在检测宫颈病变中的临床价值。 方法：选择50例HPV？？16感染患者的宫颈脱落细胞学标本，其中CIN1 11例、CIN2 11例、CIN3 21例、宫颈鳞癌（SCC）7例，另取11例病理检测HPV？？16阴性或慢性炎症者为对照。采用甲基化敏感性高分辨溶解曲线法检测HPV？？16 L1甲基化水平，采用bDNA技术检测HPV E6/E7 mRNA的表达情况。 结果：对照组、CIN1组、CIN2组、CIN3组和SCC组HPV？？16 L1甲基化阳性率分别为9.1%、72.7%、81.8%、90.5%、100.0%，HPV E6/E7 mRNA阳性率分别为45.5%、72.7%、90.9%、100.0%、100.0%；CIN2以上病变中HPV？？16 L1甲基化阳性率均高于对照组，而CIN3以上病变中HPV E6/E7 mRNA阳性率均高于对照组（P均<0.005）。HPV？？16 L1甲基化程度和HPV E6/E7 mRNA水平均随宫颈病变严重程度的增加而升高（rS=0.638和0.525，P均<0.001）；HPV？？16 L1甲基化程度与HPV E6/E7 mRNA表达水平呈正相关(rS=0.420, P=0.001)。结论：HPV？？16 L1甲基化与HPV E6/E7 mRNA检测在分流HPV感染或细胞学阳性患者方面具有良好的临床应用前景。<br>Aim: To evaluate the clinical value of HPV？？16 L1 methylation and HPV E6/E7 mRNA testing for the detection of cervical lesions.Methods: Fifty cervical cytology samples（11 cases of CIN1, 11 cases of CIN2, 21 cases of CIN3 and 7 cases of SCC）. Another 11 samples with histology？？negative or chronic inflammation were used as control. The HPV？？16 L1 methylation and HPV E6/E7 mRNA levels were detected using MS？？HRM assay and bDNA technique, respectively.Results: The overall positive rates of HPV？？16 L1 methylation and HPV E6/E7 mRNA for control, CIN1,CIN2,CIN3 and SCC groups were 9.1%,72.7%,81.8%,90.5%,100.0% and 45.5%,72.7%,90.9%,100.0%,100.0%, respectively. The HPV？？16 L1 methylation positive rate in CIN2+ groups was higher than that of control group, while the HPV E6/E7 mRNA positive rate in CIN3+ groups was higher than that of control group(all P<0.005). The HPV？？16 L1 methylation degree and HPV E6/E7 mRNA level increased with the severity of cervical lesions(rS=0.638,0.525, all P<0.001). Additionally, the HPV？？16 L1 methylation and HPV E6/E7 mRNA level had positive correlation (rS=0.420,P=0.001).Conclusion: The HPV？？16 L1 methylation and HPV E6/E7 mRNA testing may have good applied prospect for the triage of women with HPV？？infection or cytology？？positive in clinical practice
%K 人乳头瘤病毒
%K 宫颈病变
%K HPV？？16 L1甲基化
%K HPV E6/E7 mRNA&lt
%K br&gt
%K human papillomavirus
%K cervical lesion
%K HPV？？16 L1 methylation
%K HPV E6/E7 mRNA
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201601012