%0 Journal Article
%T COPD大鼠原代肺动脉平滑肌细胞中MCP-1、TGF-β1和TLR4蛋白的检测*<br>Detection of MCP-1,TGF-β1 and TLR4 protein in primary pulmonary artery smooth muscle cells of COPD rats
%A 蒋
%A 明
%A 王昌明
%A 韩旭惠
%A 杨
%A 丹
%A 马礼兵
%A 陈
%A 峰
%A 吕
%A 倩
%J 郑州大学学报（医学版）
%D 2017
%R 10.13705/j.issn.1671-6825.2017.05.031
%X 目的:探讨慢性阻塞性肺疾病(COPD)大鼠远端原代肺动脉平滑肌细胞(PASMCs)中单核细胞趋化蛋白-1(MCP-1)、转化生长因子-β1(TGF-β1)的分泌情况及其与Toll样受体4(TLR4)信号通路的相关性。方法:通过脂多糖(LPS)诱导建立大鼠COPD模型,分离培养、鉴定原代大鼠PASMCs,以LPS诱导PASMCs,然后分组(对照组、LPS组、TAK-242组,LPS+TAK-242组)培养细胞,用Western blot法检测各组PASMCs中TLR4的表达,ELISA法检测细胞培养上清液MCP-1、TGF-β1的浓度,并进行相关性分析。结果:LPS组较对照组PASMCs中TLR4的表达水平上调(P<0.05),细胞培养上清液中MCP-1、TGF-β1的含量升高(P<0.05); TAK-242组PASMCs中TLR4的表达水平下调(P<0.05),上清液中MCP-1、TGF-β1与TLR4无相关性。结论:LPS可诱导大鼠原代PASMCs合成分泌MCP-1、TGF-β1,同时上调TLR4表达水平。<br>Aim: To probe into the secretion of monocyte chemoattractant protein 1(MCP-1),transforming growth factor β1(TGF-β1)in primary pulmonary artery smooth muscle cells(PASMCs)of chronic obstructive pulmonary disease(COPD)rats and the correlation with toll-like receptor 4(TLR4)signaling pathways.Methods: The COPD rat model was established, and the PASMCs were separated, induced by LPS, and then divided into control group,LPS group,TAK-242 group,and LPS+TAK-242 group. The expression of TLR4 in PASMCs of each group was detected by Western blot, and the concentrations of MCP-1 and TGF-β1 in cell culture fluid were determined by ELISA, and at last a correlation analysis was conducted.Results: In contrast with the control group, the expression of TLR4 in PASMCs of the LPS group was inecreased(P<0.05)and the concentrations of MCP-1 and TGF-β1 in cell culture fluid were increased(P<0.05). The expression of TLR4 in PASMCs of the TAK-242 group was down-regulated(P<0.05)and the concentrations of MCP-1 and TGF-β1 in cell culture fluid showed no correlation with the expression of TLR4.Conclusion: LPS can induce PASMCs of rats to synthesize and secrete MCP-1 and TGF-β1, and the TLR4 is up-expressed at the same time
%K 慢性阻塞性肺疾病
%K Toll样受体4
%K 单核细胞趋化蛋白-1
%K 转化生长因子-β1
%K 大鼠&lt
%K br&gt
%K chronic obstructive pulmonary disease
%K Toll like receptor-4
%K monocyte chemoattractant protein 1
%K transforming growth factor β1
%K rat
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201705031