%0 Journal Article
%T Mcl-1 siRNA转染对EC9706细胞紫杉醇化疗敏感性的影响<br>Effect of Mcl-1 siRNA transfection on sensitivity of EC9706 cells to paclitaxel
%A 秦甜甜
%A 刘卫华
%A 张雪燕
%A 李蕾蕾
%A 霍俊峰
%A 石晓丽
%A 王
%A 淙
%J 郑州大学学报（医学版）
%D 2018
%R 10.13705/j.issn.1671-6825.2017.10.047
%X 目的:观察Mcl-1 siRNA转染后食管鳞癌EC9706细胞对紫杉醇敏感性的变化。方法:应用Western blot方法检测正常食管上皮Het-1细胞及EC9706细胞中Mcl-1蛋白的表达。采用不同浓度的紫杉醇分别处理转染和未转染Mcl-1 siRNA的EC9706细胞48 h,应用MTT法观察细胞增殖能力的变化; 采用0.4 μmol/L紫杉醇处理转染和未转染Mcl-1 siRNA的EC9706细胞24 h,用流式细胞术检测细胞凋亡。结果:与Het-1细胞相比,EC9706细胞中Mcl-1蛋白高表达。与单用紫杉醇处理的EC9706细胞相比,Mcl-1 siRNA转染联合紫杉醇处理的EC9706细胞增殖抑制率升高,凋亡率也升高(P<0.05)。结论:干扰抗凋亡基因Mcl-1可以增强EC9706细胞对紫杉醇的敏感性。<br>Aim: To observe the sensitivity of esophageal cancer EC9706 cells to paclitaxel(PTX)after Mcl-1 RNA interference.Methods: Western blot was used to detect the expression of Mcl-1 protein in normal esophageal epithelial Het-1 cells and esophageal carcinoma EC9706 cells. EC9706 cells transfected with or without Mcl-1 siRNA were treated with different concentrations of PTX for 48 h, and the changes in cell proliferation were observed by MTT assay. EC9706 cells transfected with or without Mcl-1 siRNA were treated with 0.4 μmol/L PTX for 24 h,and the apoptosis was examined by flow cytometry.Results: Compared with Het-1 cells, Mcl-1 protein was highly expressed in EC9706 cells. Compared with the EC9706 cells treated by PTX alone, those treated by PTX combined with Mcl-1 siRNA transfection were more sensitive to PTX with higher proliferation inhibition rate and apoptosis rate(P<0.05).Conclusion: The interference of anti-apoptosis gene Mcl-1 can increase the sensitivity of EC9706 cells to PTX
%K Mcl-1蛋白
%K EC9706细胞
%K RNA干扰
%K 紫杉醇&lt
%K br&gt
%K Mcl-1 protein
%K EC9706 cell
%K RNA interference
%K paclitaxel
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201803001