%0 Journal Article %T CUG连接蛋白1在胃癌组织中的表达及对SGC7901细胞增殖和侵袭能力的影响<br>Expression of CUG-binding protein 1 in gastric cancer tissue and its effect on SGC7901 cell proliferation and invasion %A 朱 %A 倩 %A 桂芬芳 %A 罗子华 %A 吴京华 %A 黎发明 %A 郑建伟 %J 郑州大学学报（医学版） %D 2018 %R 10.13705/j.issn.1671-6825.2018.01.014 %X 目的:探讨CUG连接蛋白1(CUGBP1)在胃癌组织中的表达及对SGC7901细胞增殖和侵袭能力的影响。方法: 收集82例胃癌和癌旁正常组织,免疫组化法检测CUGBP1蛋白。将人胃癌SGC7901细胞分为CUGBP1 siRNA组、对照siRNA组和空白对照组。转染48 h后,实时荧光定量PCR法检测细胞中CUGBP1 mRNA,MTT法检测细胞增殖能力,划痕实验检测细胞迁移能力,Transwell法检测细胞迁移和侵袭能力。结果:胃癌组织中CUGBP1蛋白阳性表达率为76.8%,高于癌旁正常组织(47.6%)(P<0.05); 胃癌组织中CUGBP1蛋白阳性表达与分化程度、TNM分期、浸润深度和淋巴结转移有关(P<0.05)。与两个对照组比较,CUGBP1 siRNA组细胞中CUGBP1 mRNA相对表达量、划痕修复率降低,迁移细胞数和侵袭细胞数减少(P<0.05)。结论:CUGBP1蛋白与胃癌的发生发展关系密切。<br>Aim: To investigate the expression of CUG-binding protein 1(CUGBP1 )in gastric cancer and its effect on cell proliferation and invasion.Methods: A total of 82 cases of gastric cancer and paracancerous tissue were selected,immunohistochemistry was used to detect the expression of CUGBP1. Human gastric cancer SGC7901 cells were divided into CUGBP1 siRNA group,control siRNA group and blank control group. After 48 h transfection, real-time fluorescence quantitative PCR was used to detect the expression of CUGBP1 mRNA in cells, MTT assay was used to detect cell proliferation, scratch test was used to detect cell migration ability, Transwell assay was used to detect cell migration and invasion.Results: The positive rate of CUGBP1 protein in gastric cancer tissue was 76.8%,which was higher than that(47.6%)in normal tissue(P<0.05). The positive rate of CUGBP1 in gastric cancer tissue was related to the degree of differentiation,TNM stage,depth of invasion and lymph node metastasis(P<0.05). Compared with those of control siRNA group and blank control group, the relative expression level of CUGBP1 mRNA in CUGBP siRNA group was lower, the wound healing rates, the number of migrating cells and the number of invasive cells were significantly reduced(P<0.05).Conclusion: CUGBP1 protein is related to the carcinogenesis and development of gastric cancer %K 胃癌 %K CUG连接蛋白1 %K 细胞增殖 %K 细胞侵袭< %K br> %K gastric cancer %K CUG-binding protein 1 %K cell proliferation %K cell invasion %U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201805010