%0 Journal Article
%T 郑州市冬季雾霾空气中细菌的多样性及耐药性检测<br>Bacterial diversity and drug resistance during winter fog-haze in Zhengzhou
%A 李梦臣
%A 刘俏丽
%A 段广才
%A 晋乐飞
%A 李
%A 影
%A 孙甜甜
%A 吴慧颖
%A 党德建
%A 刘慧莹
%A 徐亚珂
%A 陈帅印
%A 郗园林
%J 郑州大学学报（医学版）
%D 2018
%R 10.13705/j.issn.1671-6825.2018.01.027
%X 目的:了解郑州市冬季雾霾空气中细菌的种属多样性及耐药性。方法:采用ETW-6型六级筛孔撞击式微生物采样器分别对郑州市烟厂(烟厂)、郑州大学第一附属医院(一附院)、郑州纺织机械厂(郑纺机)、郑州市环保局(市环保局)4个环境监测采样点进行采样。使用营养琼脂平板培养细菌,采用改良K-B纸片法进行药敏实验。结果:2016年12月至2017年2月,于郑州市雾霾空气中共分离细菌28株(烟厂区域检出芽孢杆菌6株、假单胞菌1株; 一附院区域检出志贺菌1株、葡萄球菌1株、芽孢杆菌4株、蛾微杆菌1株; 郑纺机和市环保局区域检出芽孢杆菌各7株),其中芽孢杆菌比例最高,为24株。分离出的28株细菌对氨苄西林、庆大霉素、环丙沙星、头孢曲松、头孢哌酮、复方新诺明的耐药率依次为7.1%、0%、0%、17.9%、7.1%、0%。结论:郑州市冬季雾霾中不同地点空气中细菌的优势菌属基本相同,医院区域细菌种类较其他区域多样。雾霾空气中细菌对复方新诺明、氨苄西林、头孢哌酮、头孢曲松的耐药情况较环丙沙星、庆大霉素严重。<br>Aim: To study the species and drug resistance of bacteria in winter fog-haze in Zhengzhou.Methods: The samples were collected form 4 sampling points,Zhengzhou Cigarette Factory, the First Affiliated Hospital of Zhengzhou University, Zhengzhou Textile Machinery Factory, and Zhengzhou Municipal Environmental Protection Bureau using the ETW-6 type six level mesh impact type microbial sampler. The bacteria were cultured on a nutrient agar plate, and the antimicrobial susceptibility test was carried out by modified K-B disk method.Results: From December 2016 to February 2017, we isolated and identified 28 bacteria from the fog-haze of Zhengzhou, mainly 24 strains of Bacillus. We detected 6 strains of Bacillus and 1 strain of Pseudomonas in Zhengzhou Cigarette Factory area; there were more kinds of bacteria in the First Affiliated Hospital of Zhengzhou University, such as 1 strain of Shigella, 1 strain of Staphylococcus, 1 strain of Microbacterium, and 4 strains of Bacillus; 7 strains of Bacillus were detected in Zhengzhou Textile Machinery and Municipal Environmental Protection Bureau,respectively. The resistance rates of ampicillin, gentamicin, ciprofloxacin, ceftriaxone, cefoperazone and sulfamethoxazole were 7.1%, 0%, 0%, 17.9%, 7.1% and 0%, respectively.Conclusion: The dominant bacteria in the winter fog-haze of Zhengzhou at different locations are concordant, while the species of bacterial in the hospital area are more than in other areas. The resistance of bacteria against noxoxin, ampicillin, cefoperazone and ceftriaxone is more serious than ciprofloxacin and gentamicin in haze weather
%K 雾霾
%K 芽孢杆菌
%K 多样性
%K 耐药性&lt
%K br&gt
%K haze
%K bacillus
%K diversity
%K drug resistance
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201804028