%0 Journal Article
%T 孕妇血浆中胎儿特异性C21orf105、PLAC4 mRNA的检测*<br>Plasma levels of fetal-specific C21orf105 and PLAC4 mRNA of pregnant women with trisomy 21 syndrome fetus
%A 许雅娟△
%A 张莹莹
%A 罗晓华
%A 翟闪闪
%A 冉利敏
%A 任利单
%A 洪
%A 腾
%J 郑州大学学报（医学版）
%D 2016
%X 目的:探讨孕妇血浆中胎儿特异性C21orf105、PLAC4 mRNA的稳定性及其表达量检测的临床价值。方法:①收集30例健康单胎妊娠妇女的血样,分为8组,其中4组分别在室温中放置0、6、24、72 h,余4组分别于4 ℃条件下放置0、6、24、72 h,然后采用实时荧光定量RT-PCR法检测C21orf105、PLAC4 mRNA。②收集健康单胎妊娠妇女的血样,其中经染色体核型分析证实胎儿为21-三体综合征的孕妇38例,胎儿染色体正常的孕妇40例,同法检测血浆C21orf105、PLAC4 mRNA。结果:30例孕妇血浆中均能检测到C21orf105、PLAC4 mRNA,孕妇血浆室温放置72 h后,C21orf105、PLAC4 mRNA无明显降解(P>0.05)。21-三体综合征胎儿孕妇血浆中C21orf105、PLACA4 mRNA表达量高于正常胎儿孕妇(P<0.001)。结论:母体血浆中C21orf105、PLAC4 mRNA很稳定,其检测有望成为21-三体综合征产前筛查的有效指标。<br>Aim: To explore the stability of the fetal-specific C21orf105 and PLAC4 mRNA and their expressions in the maternal plasma of pregnant women with trisomy 21 syndrome fetus.Methods: The plasma of 30 healthy and singleton pregnant women were collected and allocated in 8 groups, 4 groups were stored for 0,6,24 and 72 h at 4 ℃, and the other 4 groups were stored for 0,6,24 and 72 h at room temperature,respectively, then the expressions of C21orf105 and PLAC4 mRNA were detected using real-time RT-PCR.A total of 40 blood samples from normal pregnant women and 38 samples from trisomy 21 syndrome fetus pregnant women were collected to detect C21orf105 and PLAC4 mRNA in maternal plasma.Results: C21orf105 and PLAC4 mRNA could be detected in all the maternal plasma samples, even in maternal plasma samples stored at room temperature for 72 h, and there was no significant differences among the 8 groups(P>0.05).The mRNA expression levels of C21orf105 and PLAC4 in maternal plasma of the trisomy 21 syndrome pregnant women were higher than those of the normal controls(P<0.001).Conclusion: C21orf105 and PLAC4 mRNA in maternal plasma is stable enough to be amplified,and they are potential markers for prenatal screening of trisomy 21 syndrome
%K 21-三体综合征
%K C21orf105
%K PLAC4
%K 血浆&lt
%K br&gt
%K trisomy 21 syndrome
%K C21orf105
%K PLAC4
%K plasma
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201602016