%0 Journal Article
%T 丙泊酚对HepG2肝母细胞瘤细胞侵袭能力的影响*<br>Effects of propofol on the invasion ability of hepatoblastoma HepG2 cells
%A 高巍
%A 宋平义
%A 景桂霞
%A 张晓琪
%A 南克俊
%A 沙保勇
%J 郑州大学学报（医学版）
%D 2015
%X 目的：探讨丙泊酚对HepG2肝母细胞瘤细胞侵袭能力的影响及相关机制。方法：用不同质量浓度（0、3、6、9和12 mg/L）丙泊酚培养HepG2细胞24 h后，通过细胞侵袭实验检测侵袭率；培养24、48和72 h后用MTT法检测细胞活性；培养48 h后采用RT？？PCR和Western blot法检测细胞中MMP？？2、MMP？？9 mRNA及MMP？？2、MMP？？9和TIMP？？1蛋白的表达。结果：随丙泊酚质量浓度的增加，HepG2细胞的侵袭能力逐渐降低（F=4 883.900，P＜0.001），细胞活性逐渐降低（F=38.334，P＜0.001），MMP？？2及MMP？？9 mRNA及蛋白的表达亦逐渐降低（P＜0.05），而TIMP？？1蛋白的表达逐渐升高（F=44.918，P＜0.001）。结论：丙泊酚能降低HepG2细胞的侵袭能力及细胞活性，其机制与抑制MMP？？2和MMP？？9蛋白的表达及促进TIMP？？1蛋白的表达有关。<br>To investigate the effects and related mechanism of propofol on the invasion ability of HepG2 cells. Methods: Different concentrations(0,3,6,9 and 12 mg/L) of propofol were used to treat HepG2 cells. The invasion ability was detected by Transwell after 24 h treatment; cell viability of HepG2 cells was studied through MTT assay after 24,48,and 72 h treatment. The expressions of MMP？？2，MMP？？9 and TIMP？？1 were measured through RT？？PCR and Western blot after 48 h treatment. Results: The invasion ability and cell viability of HepG2 cells, the mRNA and protein expressions of MMP？？2 and MMP？？9, were significantly decreased with the increase of propofol concentration(P＜0.05). The protein expression of TIMP？？1 was significantly increased with the increase of propofol concentration(F=44.918，P＜0.001). Conclusion: Propofol could reduce the invasive activity and cell viability of HepG2 cells through the inhibition of MMP？？2 and MMP？？9, and overexpression of TIMP？？1
%K 丙泊酚
%K 肝母细胞瘤
%K MMP？？2
%K MMP？？9
%K TIMP？？1
%K 侵袭&lt
%K br&gt
%K propofol
%K hepatoblastoma
%K MMP？？2
%K MMP？？9
%K TIMP？？1
%K invasion
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201503004