%0 Journal Article
%T Ets2 siRNA转染对EC9706细胞增殖、侵袭、周期和凋亡的影响<br>Effect of Ets2 siRNA transfection on proliferation,invasion,cell cycle and apoptosis of EC9706 cells
%A 杨露
%A 张楠楠
%A 张彦婷
%A 李庆华
%A 许尧
%A 朱相展
%A 薛乐勋
%A 关方霞
%J 郑州大学学报（医学版）
%D 2015
%X 目的：探讨Ets2 siRNA转染对EC9706细胞增殖、侵袭能力、周期和凋亡的影响。方法：利用Western blot检测正常食管上皮细胞系Het？？1A和食管鳞状细胞癌细胞系（EC1、Eca109、EC9706）中Ets2蛋白的表达。将EC9706细胞分为4组：阴性对照组、空白对照组、转染试剂对照组和Ets2 siRNA组，处理48 h后，采用EdU荧光标记法和CCK？？8法检测4组细胞增殖率和增殖抑制率，Transwell小室法检测细胞侵袭能力，Western blot检测E？？cadherin、Caspase？？3和Bcl？？2蛋白的表达情况，流式细胞仪检测细胞周期，Annexin V？？FITC/PI双染法检测早期细胞凋亡。结果：与Het？？1A 细胞相比，EC1、Eca109和EC9706细胞中Ets2蛋白的表达量增加（F=1 177.764，P<0.001），且EC9706细胞中增加最为显著。转染后，与阴性对照组相比，Ets2 siRNA组细胞增殖率降低、增殖抑制率升高（F=64.733和144.741，P<0.05），侵袭能力减弱（F=104.065，P<0.001），细胞周期无明显变化（P＞0.05），早期凋亡细胞增加（F=37.986，P<0.001），E？？cadherin蛋白和Caspase？？3蛋白表达增加（F=62.223和81.015，P<0.05），而Bcl？？2蛋白表达减少（F=104.439，P<0.001）。结论：Ets2下调后能有效抑制EC9706细胞增殖，降低侵袭能力，诱导凋亡；Ets2可能成为食管鳞状细胞癌的有效治疗靶点。<br>Aim: To investigate the effect of siRNA？？mediated down？？regulation of Ets2 on the proliferation, invasion, cell cycle and apoptosis of EC9706 cells in vitro. Methods: The expression of Ets2 in Het？？1A, EC1, Eca109,and EC9706 cells was analyzed by Western blot. EC9706 cells were allocated into 4 groups: negative control group, blank control group, siRNA control group, and Ets2 siRNA group. The proliferation of EC9706 cells was examined by EdU method and CCK？？8 method; the invasion was analyzed by Transwell; cell cycle was studied by flow cytometry; apoptosis was analyzed by Annexin V？？FITC/PI flow cytometry and the protein expressions of E？？cadherin,Caspase？？3 and Bcl？？2 were detected by Western blot.Results: Compared with Het？？1A cells, Ets2 showed higher expression in EC1，Eca109，and EC9706 cells（F=1 177.764，P<0.001）, especially in EC9706 cells. Compared with negative control group，the proliferation rate of EC9706 cells decreased and the inhibition rate increased significantly（F=64.733,144.741，P<0.05）. The number of invasion cells was lower in Ets2 siRNA group than that of the negative control group（F=104.065，P<0.001）. The cell cycle among the 4 groups had no obvious change(P>0.05). Compared with the negative control group , Ets2 siRNA group had a higher apoptosis rate（F=37.986，P<0.001）. The protein expression levels of E？？cadherin and Caspase？？3 increased significantly（F=62.223,81.015，P<0.05） and the Bcl？？2 protein expression level decreased obviously（F=104.439，P<0.001）. Conclusion: siRNA？？mediated down？？regulation of Ets2 could effectively inhibit the proliferation of EC9706 cells,decrease the invasion and induce the apoptosis. Therefore, Ets2 may be a potential therapeutic target for ESCC
%K Ets2
%K 增殖
%K 侵袭
%K 细胞周期
%K 凋亡
%K EC9706细胞&lt
%K br&gt
%K Ets2
%K proliferation
%K invasion
%K cell cycle
%K apoptosis
%K EC9706 cell
%U http://jms.zzu.edu.cn/oa/darticle.aspx?type=view&id=201504002