%0 Journal Article
%T 炎症小体Nod样受体蛋白3及胱天蛋白酶募集域蛋白8基因多态性与急性冠脉综合征的相关性<br>Association of NLRP3 rs10754558 and CARD8 rs2043211 polymorphisms with the occurrence of acute coronary syndrome
%A 周
%A 栋
%A 王新宏
%A 周
%A 娟
%A 樊
%A 艳
%A 万招飞
%A 刘
%A 洋
%A 袁祖贻
%J 西安交通大学学报(医学版)
%D 2016
%R 10.7652/jdyxb201601013
%X 摘要：目的?? 探讨Nod样受体蛋白3(NLRP3)rs10754558位点C>G和胱天蛋白酶募集域蛋白8(CARD8)rs2043211位点A>T多态性与急性冠脉综合征(ACS)发病的关系。 方法?? 入选450例ACS患者和380例对照组人群，使用ABI Snapshot方法分析NLRP3 rs10754558位点和CARD8 rs2043211位点多态性；冠状动脉造影后以Gensini评分评价冠脉狭窄程度；Elisa测定血清白细胞介素-1β(IL-1β)浓度。结果 NLRP3 rs10754558位点对照组和ACS组基因型频率比较差异有统计学意义 (χ2=7.64，P=0.022)，NLRP3 rs10754558位点G等位基因与ACS发病相关(AOR=1.334，95% CI=1.085～1.642，P=0.006)。而CARD8 rs2043211位点两组间基因型频率比较差异无统计学意义(χ2=1.08，P=0.582)，且与ACS发病无明显关系(AOR=1.168，95% CI=0.942～1.449，P=0.156)。ACS患者NLRP3 rs10754558位点GG基因型Gensini评分高于CC基因型(74.07±2.13 vs. 42.91±1.80，P<0.001)；IL-1β质量浓度GG基因型显著高于CC基因型[(3.21±2.68)pg/mL vs. (1.37±1.36)pg/mL, P<0.001]。 结论 NLRP3 rs10754558位点C>G基因多态性与中国汉族人群ACS发病及冠状动脉狭窄程度有关；G等位基因是ACS发病的危险等位基因，其作用与IL-1β浓度升高有关。<br>ABSTRACT: Objective?? To investigate the potential association of the Nodlike receptor protein 3 (NLRP3) rs10754558 and caspase recruitment domain-containing protein 8 (CARD8) rs2043211 SNPs with the occurrence of acute coronary syndrome (ACS). Methods?? The NLRP3 rs10754558 and CARD8 rs2043211 SNPs were analyzed using the ABI PRISM-Snapshot multiplex method in 450 Chinese Han patients with ACS and 380 controls in the case-control study. Coronary angiography was performed to evaluate the severity of coronary atherosclerosis by Gensini score. The content of IL-1β in serum was determined by ELISA. Results? ?The difference in rs10754558 allele frequency between the control group and the ACS group was significant (χ2= 7.64, P=0.022). The NLRP3 rs10754558 G allele was significantly associated with the occurrence of ACS (AOR=1.334, 95% CI=1.085-1.642, P=0.006), while CARD8 rs2043211 polymorphism was not involved (χ2=1.08, P=0.582). Patients with GG genotype of NLRP3 rs10754558 had a higher Gensini score than those with CC genotype (74.07±2.13 vs. 42.91±1.80, P<0.001). There was no significant difference in Gensini score among ACS patients with different genotypes of CARD8 rs2043211. In the ACS group, the level of IL-1β in patients with GG genotype of NLRP3 rs10754558 was significantly higher than those patients with CC genotype (3.21±2.68 vs. 1.37±1.36pg/mL, P<0.001). Conclusion?? The NLRP3 rs10754558 polymorphism is involved in the occurrence of ACS and the severity of coronary atherosclerosis in the Chinese Han population. G allele is the risk allele of ACS. The role of G allele may be associated with elevated level of IL-1β
%K 冠状动脉疾病
%K Nod样受体蛋白3(NLRP3)
%K 胱天蛋白酶募集域蛋白8(CARD8)
%K 多态性
%K 危险因素&lt
%K br&gt
%K coronary artery disease
%K Nodlike receptor protein 3 (NLRP3)
%K caspase recruitment domain-containing protein 8 (CARD8)
%K polymorphism
%K risk factor
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201601013