%0 Journal Article
%T 硒对大骨节病和骨关节炎软骨细胞超微结构的影响<br>Effects of selenium on the substructure of chondrocytes from patients with Kashin-Beck disease and osteoarthritis
%A 段
%A 琛
%A 彭
%A 侃
%A 张晓东
%A 高宗强
%A 郭
%A 雄
%J 西安交通大学学报(医学版)
%D 2016
%R 10.7652/jdyxb201601020
%X 摘要：目的 观察不同浓度硒对体外培养大骨节病(Kashin-Beck disease, KBD)、骨性关节炎(osteoarthritis, OA)和正常关节软骨细胞的超微结构变化，为大骨节病病因、发病机制研究提供科学依据。方法 依据《大骨节病诊断标准》选择Ⅱ度和Ⅲ度KBD患者8例、WOMAC诊断的OA7例和非病区正常人意外事故者5例的关节软骨进行体外分离、培养，传1代。KBD组、OA组和正常组分别给予0、0.025、0.05、0.10、0.25mg/L不同剂量的硒，采用透射电镜方法观察细胞超微结构的变化。结果 ①正常组第6天时，硒质量浓度在0.1～0.25mg/L之间组的细胞明显坏死和细胞器结构丧失，KBD组细胞超微结构细胞器丰富，状态良好，OA组细胞结构完整，细胞器存在，细胞有凋亡和坏死征象；②硒质量浓度在0.025～0.10mg/L组之间KBD、OA细胞超微结构趋于正常细胞，超微结构细胞器丰富，状态良好，而同浓度正常组细胞凋亡、坏死征象明显。③硒质量浓度>0.25mg/L时，KBD、OA组细胞微观结构受损，正常组细胞坏死明显。 结论 适宜的补硒对KBD、OA软骨细胞生长有一定的保护作用，而正常软骨细胞耐受力低、凋亡坏死较明显。<br>ABSTRACT: Objective To investigate the effects of selenium (Se) on the substructure of chondrocytes from articular cartilage in Kashin-Beck disease (KBD), osteoarthritis (OA) and normal one cultured in vitro so as to provide an experimental basis for exploring the pathogenesis of KBD. Methods The articular cartilage samples of II and III KBD and OA patients were selected according to the national diagnosis for KBD and WOMAC for OA. Chondrocytes of 8 KBD patients, 7 OA patients and 5 non-KBD region normal subjects who had accidents were separated and cultured ？？in vitro. Varying dosages of Se (0, 0.025, 0.05, 0.10mg/L and 0.25mg/L) were administered into chondrocytes in the three groups. Cell substructural changes were observed under the transmission electron microscope. Results ① On day 6, the chondrocytes had obvious apoptosis and lost organelle structure in 0.10-0.25mg/L Se groups. The substructure of chondrocytes was better in KBD groups while apoptosis and early apoptosis were seen in OA groups. ② The substructure of chondrocytes in KBD and OA groups was normal at 0.025-0.10mg/L of Se while obvious apoptosis was found in the normal groups of the same dosages. ③ The substructure of chondrocytes was damaged in KBD and OA groups with Se dosage over >0.25mg/L and obvious apoptosis was found in normal group. Conclusion Proper supplement of Se protects the growth of condrocytes in KBD and OA while those in normal group have poor tolerance and obvious apoptosis
%K 大骨节病
%K 骨关节炎
%K 硒
%K 软骨细胞
%K 超微结构&lt
%K br&gt
%K Kashin-Beck disease
%K osteoarthritis
%K selenium
%K chondrocyte
%K substructure
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201601020