%0 Journal Article
%T 辣椒素通过下调COX-2和mPGES-1抑制IL-1β诱导的NCI-H460细胞PGE？？2含量的实验研究<br>Experimental study of the inhibitory effect of capsaicin on PGE2 concentration of IL-1β induced NCI-H460 cells by downregulating COX-2 and mPGES-1
%A 任公平
%A 那
%A 辉
%A 佟
%A 雷
%A 李华洋
%J 西安交通大学学报(医学版)
%D 2016
%R 10.7652/jdyxb201602028
%X 摘要：目的 观察辣椒素对IL-1β诱导的人肺腺癌NCI-H460细胞PGE2含量的影响，并且进一步观察其对COX-2和mPGES-1的影响，探讨其抗非小细胞肺癌(NSCLC)的可能机制。方法 体外培养NCI-H460细胞，采用MTT比色分析法，观察辣椒素对NCI-H460细胞增殖抑制作用，计算IC50；采用IL-1β刺激的方法构建炎症模型，ELISA法检测辣椒素对NCI-H460细胞PGE2含量和COX-2活性的影响；Western blot法检测辣椒素对NCI-H460细胞COX-2、mPGES-1蛋白表达的影响；Real-time PCR法检测辣椒素对NCI-H460细胞COX-2 mRNA和mPGES-1 mRNA表达的影响。结果 MTT比色分析结果表明，辣椒素对NCI-H460细胞增殖具有明显抑制作用，与对照组比较，差异具有统计学意义(P<0.05或P<0.01)。辣椒素明显降低NCI-H460细胞COX-2活性和PGE2浓度，而且明显降低NCI-H460细胞COX-2、mPGES-1蛋白及其mRNA的表达，且呈剂量依赖性，与模型组比较，差异具有统计学意义(P<0.05)。结论 辣椒素通过降低NCI-H460细胞COX-2和mPGES-1 mRNA表达从而抑制PGE2释放，可能是其抗NSCLC的机制之一。<br>ABSTRACT: Objective To observe the effects of capsaicin on PGE2 concentration of IL-1β-induced human large cell carcinoma NCI-H460 cells, and further observe its effect on COX-2 and mPGES-1 so as to explore the possible mechanisms against non-small cell lung cancer. Methods NCI-H460 cells were cultured in vitro; the effect of capsaicin in inhibiting NCI-H460 cells proliferation was observed. The 50% inhibitory concentration (IC50) was measured by MTT assay. IL-1β stimulation method was used to construct inflammation model, and the effects of capsaicin on COX-2 activity and PGE2 concentration in NCI-H460 cells were measured by ELISA. The effects of capsaicin on COX-2 and mPGES-1 protein level in NCI-H460 cells were analyzed by Western blot; the effects of capsaicin on COX-2 mRNA and mPGES-1 mRNA expressions in NCI-H460 cells were analyzed by Real-time PCR. Results MTT assay results showed that the growth of NCI-H460 cells treated with capsaicin was significantly inhibited compared with the control group (P<0.05 or P<0.01). Capsaicin could significantly decrease COX-2 activity and PGE2 concentration in NCI-H460 cells, and significantly decrease COX-2, mPGES-1 protein levels as well as COX-2, mPGES-1 mRNA expressions in NCI-H460 cells in a dose-dependent manner compared with the control group (P<0.05). Conclusion Capsaicin inhibits the release of PGE2 by downregulating COX-2 and mPGES-1 mRNA expressions in NCI-H460 cells, which may be one mechanism of its effect against non-small cell lung cancer
%K 辣椒素
%K 非小细胞肺癌
%K 环氧化酶-2(COX-2)
%K 微粒体型前列腺素E合成酶-1(mPGES-1)
%K 前列腺素E2(PGE2)&lt
%K br&gt
%K capsaicin
%K non-small cell lung cancer
%K cyclooxygenase-2 (COX-2)
%K microsomal prostaglandin E synthase-1 (mPGES-1)
%K prostaglandin E2(PGE2)
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201602028