%0 Journal Article
%T 自杀行为中5-HT基因和GAD1基因的交互作用<br>Gene-gene interactions of suicidal behavior by multifactor dimensionality reduction analysis: Do both 5-HT and GAD1 work?
%A 黄缤慧
%A 于振剑
%A 冯小键
%A 董树平
%A 褚成静
%A 马文有
%J 西安交通大学学报(医学版)
%D 2018
%R 10.7652/jdyxb201801014
%X 摘要：目的 初步探讨5-羟色胺受体2C(5-HTR2C)基因、单胺氧化酶A(MAOA)基因和谷氨酸脱羧酶(GAD1)基因多态性与自杀行为的关系，并分析基因-基因之间的交互作用。方法 对中国人群中21例有自杀行为史病例样本和50例健康对照者进行基因型分型；采用多因子降维法进行交互作用分析。结果 对照组符合哈温平衡(HWE)检验，病例组中5-HTR2C位点不符合HWE检验(P<0.05)，经过性别矫正检验显示与自杀行为正相关；两组的5-HTR2C基因型频率差别有统计学意义(χ2=6.18，P=0.04)；病例组和对照组MAOA、GAD1基因型频数差异均无显著统计学意义(P>0.05)。在MDR模型分析得到最优模型组合为rs5953210-rs769391位点构建的交互模型OR=20.19，95% CI 4.19～97.38，P<0.01，交互作用显著。结论 5-HTR2C 基因rs3813928、rs518147位点多态性可能与自杀行为存在关联，与GAD1基因rs3749034、rs769391位点和MAOA基因rs5953210、rs979605位点无关联。在MDR模型中，5-HTR2C 基因与MAOA基因在自杀行为发病中具有显著交互效应，可增加自杀行为的发病风险。<br>ABSTRACT: Objective To investigate gene-gene interactions of suicidal behavior with single-nucleotide-polymorphism (SNP) in MAOA, GAD1 and 5-HTR2C by multifactor dimensionality reduction. Methods For this case-control study, six SNPs were captured in related genes and detected in blood samples obtained from 21 patients with suicidal behavior and 50 healthy individuals. The genotype frequency and allele frequency as well as the Hardy-Weinberg equilibrium (HWE), tests were performed and compared by plink software. The gene-gene interactions models were built by the MDR software. Results The HWE test for case group showed that rs3813928 rs518147 of 5-HTR2C gene was not in line with HWE (P<0.05). However, the additive model analysis after adjustment by gender indicated that the polymorphism had a positive correlation with suicidal behavior in case group. The case and control groups differed significantly only in genotype frequencies of 5-HTR2C gene (χ2=6.18, P=0.04). There was no significant difference in allele and genotype frequencies of the other genes (P>0.05). The best combination model of MDR was rs5953210-rs769391 OR=20.19, 95% CI 4.19-97.38, P<0.01, with significant interaction. Conclusion The 5-HTR2C gene rs3813928 and rs518147 polymorphisms may play an important role in the susceptibility to suicidal behavior. The combination of MAOA with GAD1 has a significant interaction which may increase the risk of suicidal behavior
%K 自杀行为
%K 多因子降维法
%K 5-HT受体2C基因
%K 单胺氧化酶A
%K γ-氨基丁酸
%K 谷氨酸脱羧酶交互分析&lt
%K br&gt
%K suicidal behavior
%K multifactor dimensionality reduction (MDR)
%K 5-HTR2C
%K MAOA
%K γ-GABA
%K GAD1
%K gene-gene interaction
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201801014