%0 Journal Article
%T 蛛网膜下腔出血大鼠海马区A-β蛋白表达与认知功能障碍的关联研究<br>Expression of A-β protein in the hippocampus of rats with subarachnoid hemorrhage and its correlation with cognitive dysfunction
%A 何
%A 娟
%A 吴
%A 琼
%A 李
%A 娜
%A 郝璞珩
%J 西安交通大学学报(医学版)
%D 2018
%R 10.7652/jdyxb201802017
%X 摘要：目的 研究蛛网膜下腔出血(subarachnoid hemorrhage, SAH)后海马区A-β蛋白表达增加对大鼠学习及记忆功能的影响。方法 将48只SD大鼠随机分为模型组和假手术组，每组24只。采用Bederson法建立SAH模型，分别于造模后第3、7、14、28天时采用Morris水迷宫检测大鼠空间学习及记忆功能，并用免疫组化法检测海马区A-β蛋白的表达。结果 造模后相应时间点，模型组比假手术组逃避潜伏期时间明显延长(P<0.05 )；模型组与假手术组在3d时A-β蛋白表达无明显差异(P>0.05)，在7、14、28d时，A-β阳性细胞计数明显增加(P<0.05)。结论 大鼠SAH后出现学习记忆功能减退及A-β蛋白表达增加，可能促使认知功能障碍的发生。<br>ABSTRACT: Objective To study the effect of increased brain edema and the expression of A-β protein in the hippocampus on learning and memory functions of rats after subarachnoid hemorrhage (SAH). Methods We randomly divided 48 adult SD rats into model group and sham-operation group, each with 24. The model of SAH was established by Bederson method. Morris water maze was used to detect the spatial learning and memory functions of the rats at day 3, 7, 14 and 28 after modeling; A-β protein expression in the hippocampus was detected by the immunohistochemical method. Results At the corresponding time points after modeling, the latency of escape was significantly longer in model group than that in sham-operation group (P<0.05). However, the two groups did not significantly differ in A-β protein expression at day 3 (P>0.05); A-β positive cell counts increased significantly at days 7, 14 and 28 (P<0.05). Conclusion Memory impairment of learning machine, edema of brain tissue, and the increase of A-β protein expression may induce cognitive dysfunction after SAH
%K 蛛网膜下腔出血
%K 脑组织水肿
%K A-β蛋白
%K 认知功能障碍
%K 大鼠&lt
%K br&gt
%K subarachnoid hemorrhage
%K brain edema
%K amyloid-β (A-β) peptide
%K cognitive dysfunction
%K rat
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201802017