%0 Journal Article
%T vIL-10对鼻咽癌细胞株MHC-1类分子抗原加工提呈“操纵子”表达的影响<br>Effects of vIL-10 on MHC-I antigen processing "the operon" in nasopharyngeal carcinoma cell lines
%A 任艳鑫
%A 杨
%A 洁
%A 孙瑞梅
%A 赵留芳
%A 李
%A 磊
%A 张世文
%A 费继敏
%A 尚艺泰
%A 李舟蕾
%A 李晓江
%J 西安交通大学学报(医学版)
%D 2016
%R 10.7652/jdyxb201604011
%X 摘要：目的 研究病毒白介素-10(vIL-10)对鼻咽癌细胞株MHC-1类分子抗原加工提呈“操纵子”表达的影响。方法 收集不同时间段vIL-10处理的鼻咽癌细胞(CNE-1和CNE-2)，采用RT-PCR、Western blot方法检测vIL-10处理的鼻咽癌细胞中MHC-1类分子抗原加工提呈“操纵子”(TAP-1、2，LMP-2、7及HLA-I)表达的变化。结果 ①mRNA水平：CNE-1和CNE-2的TAP-1水平在不同时间点均未表现出显著差异；vIL-10作用1、4、6、12h时，CNE-1和CNE-2的TAP-2、LMP-2均未出现变化，24h时，两者均出现显著下降甚至消失；CNE-1在vIL-10作用后4h即出现LMP-7下降，而CNE-2在vIL-10作用后6h出现下降；在vIL-10作用24h时CNE-1和CNE-2的HLA-I出现显著下降。②蛋白水平：vIL-10作用后24h时，两种细胞的TAP-1均出现显著下降，TAP-2则在vIL-10作用后1、6、12、24h出现逐渐下降；CNE-2的LMP-2、LMP-7在vIL-10作用后随时间逐渐下降，而CNE-1的两种蛋白仅在作用后12h出现显著下降；HLA-I在vIL-10作用后出现下降趋势，但在CNE-1中各时间段变化无统计学差异，CNE-2在24h显著下降。结论 vIL-10可明显抑制鼻咽癌MHC-I类分子抗原加工和提呈的“操纵子”的表达，并且呈一定的时间依赖性。<br>ABSTRACT: Objective To explore the effects of virus interleukin-10(vIL-10) on different expressions of MHC-I antigen processing "the operon". Methods We collected nasopharyngeal carcinoma cells (CNE-1 and CNE-2) treated by vIL-10 at different time points, and detected the changes of MHC-I antigen processing “the operons” (TAP-1, TAP-2，LMP-2, LMP-7 and HLA-I) by RT-PCR and Western blot. Results ① mRNA level: There was no difference in the expression of TAP-1 in CNE-1 and CNE-2 cells at various time points. The expressions of TAP-2 and LMP-2 in CNE-1 and CNE-2 did not change at 1, 4, 6, 12h, but downregulated and even disappeared at 24h. The expression of LMP-7 in CNE-1 decreased 4h after vIL-10 was added, and that in CNE-2 decreased at 6h. The expression of HLA-I in CNE-1 and CNE-2 showed significant decrease at 24h. ② Protein expression: The expression of TAP-1 in CNE-1 and CNE-2 showed significant decrease at 24h. The expression of TAP-2 in CNE-1 and CNE-2 was gradually downregulated at different time points. The expressions of LMP-2 and LMP-7 in CNE-2 were gradually downregulated at different periods, while that in CNE-1 was only decreased at 12h. The expression of HLA-I in CNE-1 and CNE-2 was gradually downregulated, but there was no significant difference at each period in CNE-1, while the expression of HLA-I in CNE-2 at 24h was significantly downregulated. Conclusion vIL-10 can inhibit the expression of MHC-I antigen processing "the operon" in NPC in the time-dependent manner
%K 鼻咽肿瘤
%K 病毒白介素-10
%K 抗原处理相关转运体蛋白(TAP)
%K 低分子量多肽复合物(LMP)
%K 人类白细胞抗原-I(HLA-I)&lt
%K br&gt
%K nasopharyngeal carcinoma (NPC)
%K virus interleukin-10
%K transporter associated with antigen processing (TAP)
%K low molecular mass polypeptide (LMP)
%K human leukocyte antigen I (HLA-I)
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201604011