%0 Journal Article
%T 苏子油干预血管内皮细胞miR-21靶向调控MMP-9的机制<br>Mechanism of Perilla oil’s intervention in vascular endothelial cell miR-21 targeted regulation of MMP-9
%A 黄楦槟
%A 陈金水
%A 吴天敏
%A 陈金雄
%J 西安交通大学学报(医学版)
%D 2016
%R 10.7652/jdyxb201601024
%X 摘要：目的 探讨不同浓度苏子油干预人脐静脉血管内皮细胞(HUVEC) miR-21和基质金属蛋白酶MMP-9的表达，为苏子油改善动脉粥样硬化提供依据。方法 体外培养HUVEC，以TNF-α诱导细胞进行造模，将细胞分为空白组、模型组及苏子油高、中、低剂量组。MMT法检测各组细胞A值；PT-PCR检测细胞miR-21、MMP-9基因水平；脂质体转染法将miR-21模拟物转入HUVEC细胞，Western blot检测转染后MMP-9蛋白的表达。结果 苏子油高、中、低剂量能明显改善TNF-α诱导的HUVEC损伤，且苏子油对内皮细胞的保护作用呈剂量依赖关系；RT-PCR结果显示，模型组miR-21及MMP-9的表达高，苏子油干预后，miR-21及MMP-9基因的表达逐渐减弱，且二者分别与模型组比较，差异有统计学意义(P<0.05)；细胞转染miR-21后，MMP-9蛋白的表达显著增强；miR-21与MMP-9 mRNA 3′UTR区经生物信息学软件预测存在靶向结合位点。结论 苏子油可能通过干预miR-21靶向调控MMP-9的表达对血管内皮细胞发挥保护作用，从而发挥抗动脉粥样硬化作用。<br>ABSTRACT: Objective To explore the intervention of different concentrations of Perilla oil in miR-21 and matrix metalloproteinase 9 (MMP-9) expressions of human umbilical vein endothelial cells (HUVECs) to provide the basis for Perilla oil？？s improvement of atherosclerosis. Methods In vitro umbilical vein endothelial cells were induced by TNF-α, and then divided into blank group, model group as well as Perilla oil high-, medium- and low-dose groups. A value was detected by MMT method, and gene levels of cell miR-21 and MMP-9 were detected by PT-PCR. Liposome transfection method was used to put the miR-21 analog into HUVEC cells; the expression of MMP-9 protein after transfection was detected by Western blot. Results Perilla oil of three doses could obviously improve HUVEC injury induced by TNF-α, and Perilla oil？？s protection of endothelial cells was dose-dependent. miR-21 and MMP-9 gene expressions detected by RT-PCR showed that both of them were higher in model group. After Perilla oil intervention, the expression of miR-21 and MMP-9 gene gradually decreased with the dose of Perilla oil, both of which differed significantly from those in model group (P<0.05). After cell transfection of miR-21, the expression MMP-9 protein was significantly increased. MiR-21 and MMP-9 mRNA 3？？UTR region detected by bioinformatics software could predict target binding site. Conclusion Perilla oil can protect vascular endothelial cells by intervening in miR-21？？s regulation of MMP-9 expression, thus having anti-atherosclerosis effect
%K 苏子油
%K 微小RNA-21
%K 基质金属蛋白酶-9
%K 靶向调控
%K 抗动脉粥样硬化&lt
%K br&gt
%K Perilla oil
%K miR-21
%K MMP-9
%K targeted control
%K anti-atherosclerosis
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201601024