%0 Journal Article
%T 藏红花通过抗凋亡、抗炎和抗水肿机制发挥对大鼠脊髓损伤模型的神经保护作用<br>Neuroprotective effects of safranal on a rat model of traumatic injury to the spinal cord by anti-apoptotic, anti-inflammatory and edema-attenuating actions
%A 张
%A 晨
%A 吕雷锋
%A 李
%A 苗
%A 高
%A 玲
%A 马
%A 骏
%A 王坤正
%J 西安交通大学学报(医学版)
%D 2017
%R 10.7652/jdyxb201702024
%X 摘要：目的 研究藏红花对大鼠脊髓损伤后神经功能的保护作用、相关病理变化及其作用机制。方法 36只大鼠随机均分为6组：正常组、损伤组及治疗组A、B、C、D。通过BBB评分法(the Basso Beattie Bresnahan locomotor rating scale)和HE染色评估藏红花的神经保护作用并确定最有效剂量。另取60只大鼠随机均分为3组：正常组、损伤组、治疗组，通过尼氏染色、TUNEL染色及电子显微镜观察评估组织病理学变化；RT-PCR、免疫组化检测凋亡相关蛋白Bax与Bcl-2的表达；ELISA、Western blot、免疫组化等检测炎症相关因子1L-1β、1L-10、TNF-α、P38MAPK的表达，Western blot、免疫组化检测水肿相关因子APQ-4的表达。结果 藏红花最有效剂量为100mg/kg。治疗组与损伤组相比，神经细胞结构更清晰，凋亡细胞数目显著降低(26.37±1.54 vs. 35.94±1.62，P=0.000)，Bax表达明显降低(P=0.000)而Bcl-2表达有所提高(P=0.036)，P38MAPK表达水平明显降低[(300.30±33.26)% vs. (132.54±10.21)%，P=0.000]，APQ-4表达水平降低[(359.55±16.12)% vs. (124.53±20.35)%，P=0.000]。结论 藏红花通过抗凋亡、抗炎及抗水肿机制在大鼠脊髓损伤后起到神经保护的作用。<br>ABSTRACT: Objective To investigate the effect of safranal on neurologic functions and histopathologic changes after spinal cord injury (SCI) and the related molecular mechanisms. Methods We randomly assigned 36 rats into six groups: control group, injury group and four treatment groups (namely, A, B, C, and D). The Basso Beattie Bresnahan locomotor rating scale (BBB) and HE staining were applied to evaluate the neuroprotective effect and determine the most effective dosage. Another 60 rats were randomly and evenly assigned to three groups: control group, injury group and treatment group. Nissl staining, TUNEL staining and electron microscopy were used to analyze histopathological changes; RT-PCR, immunohistopathological staining, ELISA, and Western blot were used to detect the expressions of apoptosis-related proteins (Bax and Bcl-2), inflammation-related factors (IL-1β, IL-10, TNF-α and P38MAPK), and edema-related factor (APQ-4). Results The optimal dosage for safranal was 100mg/kg. Neurocyte structure was found more distinct in treatment group than in injury group. In addition, we detected a smaller number of apoptotic neurocyte (26.37±1.54 vs. 35.94±1.62, P=0.000), decreased Bax (P=0.000) and APQ-4[(359.55±16.12)% vs. (124.53±20.35)%, P=0.000] expressions, increased Bcl-2 (P=0.036) expression, and obviously lowered P38MAPK [(300.30±33.26)% vs. (132.54±10.21)%, P=0.000] expression. Conclusion Safranal exerts its neuroprotective function through anti-apoptosis, anti-inflammation and anti-edema in the rat model of spinal cord injury
%K 藏花醛
%K 神经保护
%K Bax
%K P38MAPK
%K APQ-4
%K Bcl-2
%K 脊髓损伤&lt
%K br&gt
%K safranal
%K neuroprotection
%K Bax
%K P38MAPK
%K APQ-4
%K Bcl-2
%K spinal cord injury
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201702024