%0 Journal Article
%T miR-200b对三阴性乳腺癌细胞MDA-MB-231的负性调控作用<br>miRNA-200b suppresses triple negative breast cancer cells in vitro
%A 刘
%A 棣
%A 张寅斌
%A 闫婉君
%A 赵小瑶
%A 盛倩文
%A 许
%A 朋
%A 管海涛
%A 张淑群
%J 西安交通大学学报(医学版)
%D 2018
%R 10.7652/jdyxb201805026
%X 摘要：目的 研究miRNA-200b对三阴性乳腺癌细胞增殖、侵袭能力的影响。方法 定量PCR检测人乳腺癌细胞株MCF-7、BT-474、MDA-MB-453、SKBR3、MDA-MB-468、MDA-MB-231中miRNA-200a/b/c的表达情况，通过miR-200b抑制剂及拟似物分别抑制、促进miR-200b的表达后，观察癌细胞增殖能力、克隆形成及侵袭能力的变化。结果 miRNA-200家族在Luminal型乳腺癌细胞MCF-7、BT-474和Her-2表达型MDA-MB-453、SKBR3的表达量高于三阴性型MDA-MB-468、MDA-MB-231的表达。通过inhibitor、mimics分别抑制、促进MDA-MB-231细胞中miR-200b的表达后，结果显示miR-200b对肿瘤细胞的增殖有抑制作用(P<0.05)，miRNA-200b组对肿瘤细胞的克隆形成有抑制作用(P<0.05)，对癌细胞侵袭有抑制作用(P<0.05)。结论 miRNA-200b对三阴性乳腺癌细胞MDA-MB-231具有负性调控作用，可以作为潜在的早期诊断及治疗靶点。<br>ABSTRACT: Objective To identify the regulation of miRNA-200b on proliferation of triple negative breast cancer cells. Methods The expressions of miRNA-200a/b/c in breast cancer cell lines MCF-7, BT-474, MDA-MB-453, SKBR3, MDA-MB-468, and MDA-MB-231 were detected by real-time PCR. MTT was used to detect MDA-MB-231 cell proliferation after miR-200b was downregulated and upregulated. The colony-forming efficiency of miR-200b was detected by colony forming assay. The transwell chamber was used to investigate the influence of miR-200b on invasion of MDA-MB-231 cells. Results miRNA-200a/b/c had relatively low expressions in triple negative breast cancer cell lines MDA-MB-468 and MDA-MB-231, but high expressions in luminal cells MCF-7, BT-474, and Her-2 positive cells MDA-MB-453, SKBR3. The upregulated miR-200b could suppress the proliferation, colony formation and cell invasion of MDA-MB-231 cells (P<0.05). Also the downregulated miR-200b promoted cell proliferation, colony forming and cell invasion (P<0.05). Conclusion miRNA-200b suppresses triple negative breast cancer cell MDA-MB-231, making it a potential biomarker and target for early diagnosis and therapy
%K miRNA
%K 乳腺癌
%K miR-200b&lt
%K br&gt
%K miRNA
%K breast cancer
%K miR-200b
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201805026