%0 Journal Article
%T 激光人工皱缩对囊胚玻璃化冷冻后妊娠结局的影响<br>Effect of laser artificial shrinkage on pregnancy outcome in vitrification of human expanded blastocysts
%A 任文娟
%A 师娟子
%A 李明昭
%A 张四林
%A 薛
%A 侠
%A 赵皖秋
%A 洪慧慧
%A 周党侠
%J 西安交通大学学报(医学版)
%D 2018
%R 10.7652/jdyxb201802025
%X 摘要：目的 探讨囊胚激光人工皱缩预处理方法(LAS)在人囊胚玻璃化冻融中的应用效果。方法 选取2014年1月1日至2015年12月31日期间的患者行解冻囊胚周期3859例。根据是否进行激光人工皱缩将其分为激光皱缩组(3176例)和未皱缩组(683例)，进一步根据不同年龄分为<36岁组和≥36岁组，主要比较指标：复苏率、临床妊娠率、种植率。结果 激光皱缩组的复苏率、临床妊娠率、种植率分别为97.32%(5453/5603)、66.81%(2118/3170)、53.55%(2912/5438)；未皱缩组的复苏率、临床妊娠率、种植率分别为95.13%(1173/1233)、62.70%(427/681)、49.74%(582/1170)；二组相比均有统计学差异(P<0.05)。进一步的亚组分析显示，<36岁激光皱缩组的复苏率高于未皱缩组[97.27%(4887/5024) vs. 95.33%(1040/1091)] (P<0.05)。≥36岁激光皱缩组的复苏率、生化妊娠率高于对照组[97.75%(566/579) vs. 93.66%(133/142)]、[65.45%(216/330) vs. 50.65%(39/77)] (P<0.05)。激光皱缩组和对照组的取消移植率分别为0.19%和0.29%，两组比较差异无统计学意义(P>0.05)。结论 冷冻前采用激光人工皱缩技术可提高囊胚解冻后的复苏率、临床妊娠率、种植率。<br>ABSTRACT: Objective To investigate the effect of laser artificial shrinkage (LAS) on pregnancy outcome in vitrification of human expanded blastocysts. Methods We selected 3859 frozen-thawed blastocyst-stage embryo transfers from January 2014 to December 2015. The transfers were divided into LAS group (n=3176) and non-LAS group (n=683), which were then subdivided into <36y subgroup and ≥36y subgroup according to their age. Main outcomes measures were thawing rate, implantation rate and clinical pregnancy rate. Results Thawing rate, clinical pregnancy rate and implantation rate were 97.32% (5453/5603), 66.81% (2118/3170), and 53.55% (2912/5438) in LAS group. In non-shrink group, they were 95.13% (1173/1233), 62.70% (427/681), and 49.74% (582/1170), which did not significantly differ from those in the former group (P<0.05). Further analysis of the subgroups showed that thawing rate was significantly higher in LAS group than in non-shrink group of patients <36y (97.27% vs. 95.33%; P<0.05). Thawing rate and biochemical pregnancy rate were significantly higher in LAS group than in non-shrink group in patients ≥36y (97.75% vs. 93.66%; 65.45% vs. 50.65%, P<0.05). Cancellation rate was not significantly different between the two groups (0.19% vs. 0.29%, P>0.05). Conclusion LAS technique can increase thawing rate, clinical pregnancy rate and implantation rate before cryopreservation of blastocysts
%K 囊胚
%K 激光人工皱缩
%K 玻璃化冷冻
%K 妊娠结局&lt
%K br&gt
%K blastocyst
%K laser artificial shrinkage
%K vitrification freeze
%K pregnancy outcome
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201802025