%0 Journal Article
%T IL-1β和MMP-13在兔骨关节炎模型软骨和滑液中的表达<br>Expressions of interleukin-1 beta and matrix metalloproteinase-13 in articular cartilage and synovial fluid of osteoarthritis model in rabbits
%A 黄媛霞
%A 徐海斌
%A 郭
%A 春
%J 西安交通大学学报(医学版)
%D 2017
%R 10.7652/jdyxb201704008
%X 摘要：目的 评价兔膝关节内侧半月板损伤制作骨关节炎(OA)模型的方法的可行性，探讨白细胞介素-1β(IL-1β)和金属蛋白酶-13(MMP-13)在兔骨关节炎模型软骨和滑液病变中的作用机制。方法 新西兰大白兔40只，随机分为实验组(n=30)和对照组(n=10)，于术后2、6、12周观察股骨髁关节软骨的病理变化并进行评分；用免疫组化方法检测关节软骨中IL-1β和MMP-13的表达情况；用酶联免疫吸附法测定关节液中IL-1和MMP-13的含量。结果 实验组和对照组在大体和病理观察不同时间点关节软骨评分及HE染色差异均有统计学意义(P<0.05)。免疫组织化学检测结果显示IL-1β在两组中均有表达，实验组和对照组2周时细胞分数差异无统计学意义，6、12周差异有统计学意义(P<0.05)。MMP-13在对照组关节软骨细胞中未见表达，实验组的关节软骨细胞中可见MMP-13蛋白的表达，差异有统计学意义(P<0.05)。关节滑液中IL-1β表达和在软骨中表达一致。结论 采用损伤兔膝关节半月板的方法可建立合理的动物OA模型；IL-1β和MMP-13在OA发病过程中表达水平有明显变化，需要进一步的临床研究来探讨其是否可以作为早期诊断OA的指标之一。<br>ABSTRACT: Objective To investigate the feasibility of osteoarthritis (OA) model in rabbits by injuring medial meniscus so as to understand the role of interleukin-1 beta (IL-1β) and matrix metalloproteinase-13 (MMP-13) in pathological mechanism of articular cartilage and synovial fluid of OA model in rabbits. Methods We randomly divided 40 New Zealand white rabbits into experimental group (n=30) and control group (n=10). Pathological changes in articular cartilage of the femoral condyle were scored at weeks 2, 6 and 12 after surgery. We detected the expressions of IL-1β and MMP-13 by immunohistochemistry. The cell fractions of IL-1β and MMP-13 were recorded by ELISA. Results The articular cartilage score and HE staining significantly differed at various time points of gross and pathological observation between control group and experimental group (P<0.05). Immunohistochemistry showed that IL-1β was expressed in both groups and that the cell fraction differed significantly at weeks 6 and 12 ？？(P<0.05),？？ but not at week 2 between the two groups. MMP-13 protein expression was not detected in articular condrocytes in control group, but was detected in experiment group with a significant difference (P<0.05). IL-1β expression was consistent in articular cartilage and synovial fluid. Conclusion The reasonable rabbit animal OA model could be established by knee meniscus injury caused by surgical method. Expressions of IL-1β and MMP-13 change obviously in the pathomechanism of OA. Further clinical studies are needed to determine whether they can be used as markers in early diagnosis of OA
%K 骨性关节炎
%K 关节软骨
%K 关节滑液
%K IL-1β
%K MMP-13&lt
%K br&gt
%K osteoarthritis
%K articular cartilage
%K synovial fluid
%K IL-1β
%K MMP-13
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201704008